UniProt Accession

 WEE1_HUMAN; P30291; B3KVE1; D3DQV0 

Genbank Protein ID

 U10564; AJ277546; AK122837; AC011979; AC122179; CH471064; CH471064; CH471064; X62048 

Genbank Nucleotide ID

 AAB60401.1; CAC14173.1; BAG53753.1; EAW68586.1; EAW68587.1; EAW68588.1; CAA43979.1 

Protein Name

 Wee1-like protein kinase 

Protein Synonyms/Alias

 WEE1hu; Wee1A kinase 

Gene Name

 WEE1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
292	TITESNMKSRYTTEF	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. 

Sequence Annotation

 DOMAIN 299 569 Protein kinase.
 NP_BIND 305 313 ATP (By similarity).
 ACT_SITE 426 426 Proton acceptor (By similarity).
 METAL 431 431 Magnesium; via carbonyl oxygen.
 METAL 463 463 Magnesium; via carbonyl oxygen.
 BINDING 328 328 ATP (By similarity).
 MOD_RES 53 53 Phosphoserine; by PLK1.
 MOD_RES 123 123 Phosphoserine; by CDK1.
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 150 150 Phosphoserine.
 MOD_RES 190 190 Phosphothreonine.
 MOD_RES 239 239 Phosphothreonine.
 MOD_RES 312 312 Phosphoserine.
 MOD_RES 642 642 Phosphoserine; by BRSK1 and BRSK2.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transferase; Tyrosine-protein kinase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 646 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:EC.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
 GO:0004713; F:protein tyrosine kinase activity; TAS:ProtInc.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0030010; P:establishment of cell polarity; IEA:Compara.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0000226; P:microtubule cytoskeleton organization; IEA:Compara.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0048812; P:neuron projection morphogenesis; IEA:Compara.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; IEA:Compara.
 GO:0051726; P:regulation of cell cycle; TAS:Reactome. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS.
 IPR017164; Wee1-like_protein_kinase. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS