CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013502
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA pseudouridine synthase D 
Protein Synonyms/Alias
 tRNA pseudouridine(13) synthase; tRNA pseudouridylate synthase D; tRNA-uridine isomerase D 
Gene Name
 truD 
Gene Synonyms/Alias
 ygbO; b2745; JW2715 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
64FVADALAKFLKIHARacetylation[1]
81SFAGQKDKHAVTEQWacetylation[1]
293QALLVREKVEAARRAacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs. 
Sequence Annotation
 DOMAIN 155 303 TRUD.
 REGION 180 187 RNA binding (Probable).
 ACT_SITE 31 31 Proton acceptor (Potential).
 ACT_SITE 80 80 Nucleophile.
 BINDING 27 27 Substrate (By similarity).
 BINDING 129 129 Substrate (Probable).
 BINDING 329 329 Substrate (By similarity).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Isomerase; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 349 AA 
Protein Sequence
MIEFDNLTYL HGKPQGTGLL KANPEDFVVV EDLGFEPDGE GEHILVRILK NGCNTRFVAD 60
ALAKFLKIHA REVSFAGQKD KHAVTEQWLC ARVPGKEMPD LSAFQLEGCQ VLEYARHKRK 120
LRLGALKGNA FTLVLREVSN RDDVEQRLID ICVKGVPNYF GAQRFGIGGS NLQGAQRWAQ 180
TNTPVRDRNK RSFWLSAARS ALFNQIVAER LKKADVNQVV DGDALQLAGR GSWFVATTEE 240
LAELQRRVND KELMITAALP GSGEWGTQRE ALAFEQAAVA AETELQALLV REKVEAARRA 300
MLLYPQQLSW NWWDDVTVEI RFWLPAGSFA TSVVRELINT TGDYAHIAE 349 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP. 
Interpro
 IPR020103; PsdUridine_synth_cat_dom.
 IPR001656; PsdUridine_synth_TruD.
 IPR020119; PsdUridine_synth_TruD_CS.
 IPR011760; PsdUridine_synth_TruD_insert. 
Pfam
 PF01142; TruD 
SMART
  
PROSITE
 PS50984; TRUD
 PS01268; UPF0024 
PRINTS