CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002463
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein URA2 
Protein Synonyms/Alias
 Glutamine-dependent carbamoyl-phosphate synthase; Aspartate carbamoyltransferase 
Gene Name
 URA2 
Gene Synonyms/Alias
 YJL130C; J0686 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
214YVPPADNKHIELQTGacetylation[1]
214YVPPADNKHIELQTGubiquitination[2]
574EINEKCAKSQAANSVubiquitination[2]
762NIPLNEVKNSVTKSTubiquitination[2]
864AIANAFAKKGYSVDKacetylation[1]
880WEMTRIDKWFLNKLHacetylation[1]
902KISSFGTKEELPSLVacetylation[1]
902KISSFGTKEELPSLVubiquitination[2]
1080TLHRENVKILGTSPDubiquitination[2]
1180TKYIENAKEIEMDAVubiquitination[2]
1253YNIQFIAKDNEIKVIacetylation[1]
1301LTPYPVEKLPDDYVAubiquitination[2]
1357SLLATGFKLPKKNILacetylation[1]
1853ELQSTSAKRRFSITEubiquitination[2, 3]
1917GRHILSIKQFKRSDFacetylation[1]
1989NPLVSSVKKGETLQDubiquitination[3]
2158DTYIVDNKILAHAKEacetylation[1]
2180LPRVNEIKEEVDYDHacetylation[1]
2196AAYFRQMKYGLFVRMacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 This protein is a "fusion" protein encoding three enzymatic activities of the pyrimidine pathway (GATase, CPSase, and ATCase). 
Sequence Annotation
 DOMAIN 228 413 Glutamine amidotransferase type-1.
 DOMAIN 562 754 ATP-grasp 1.
 DOMAIN 1099 1290 ATP-grasp 2.
 REGION 1 400 GATase (Glutamine amidotransferase).
 REGION 401 440 Linker.
 REGION 441 1482 CPSase (Carbamoyl-phosphate synthase).
 REGION 1483 1492 Linker.
 REGION 1493 1821 Defective DHOase domain.
 REGION 1822 1909 Linker.
 REGION 1910 2214 ATCase (Aspartate transcarbamylase).
 ACT_SITE 302 302 For GATase activity (By similarity).
 ACT_SITE 386 386 For GATase activity (By similarity).
 ACT_SITE 388 388 For GATase activity (By similarity).
 MOD_RES 1857 1857 Phosphoserine; by PKA.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyrimidine biosynthesis; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2214 AA 
Protein Sequence
MATIAPTAPI TPPMESTGDR LVTLELKDGT VLQGYSFGAE KSVAGELVFQ TGMVGYPESV 60
TDPSYEGQIL VITYPLVGNY GVPDMHLRDE LVEELPRYFE SNRIHIAGLV ISHYTDEYSH 120
YLAKSSLGKW LQNEGIPAVY GVDTRSLTKH LRDAGSMLGR LSLEKSGSDR TISRSSSWRS 180
AFDVPEWVDP NVQNLVSKVS INEPKLYVPP ADNKHIELQT GPDGKVLRIL AIDVGMKYNQ 240
IRCFIKRGVE LKVVPWNYDF TKEDYDGLFI SNGPGDPSVL DDLSQRLSNV LEAKKTPVFG 300
ICLGHQLIAR AAGASTLKLK FGNRGHNIPC TSTISGRCYI TSQNHGFAVD VDTLTSGWKP 360
LFVNANDDSN EGIYHSELPY FSVQFHPEST PGPRDTEFLF DVFIQAVKEF KYTQVLKPIA 420
FPGGLLEDNV KAHPRIEAKK VLVLGSGGLS IGQAGEFDYS GSQAIKALKE EGIYTILINP 480
NIATIQTSKG LADKVYFVPV TAEFVRKVIL HERPDAIYVT FGGQTALSVG IAMKDEFEAL 540
GVKVLGTPID TIITTEDREL FSNAIDEINE KCAKSQAANS VDEALAAVKE IGFPVIVRAA 600
YALGGLGSGF ANNEKELVDL CNVAFSSSPQ VLVEKSMKGW KEVEYEVVRD AFDNCITVCN 660
MENFDPLGIH TGDSIVVAPS QTLSDEDYNM LRTTAVNVIR HLGVVGECNI QYALNPVSKD 720
YCIIEVNARL SRSSALASKA TGYPLAYTAA KLGLNIPLNE VKNSVTKSTC ACFEPSLDYC 780
VVKMPRWDLK KFTRVSTELS SSMKSVGEVM SIGRTFEEAI QKAIRSTEYA NLGFNETDLD 840
IDIDYELNNP TDMRVFAIAN AFAKKGYSVD KVWEMTRIDK WFLNKLHDLV QFAEKISSFG 900
TKEELPSLVL RQAKQLGFDD RQIARFLDSN EVAIRRLRKE YGITPFVKQI DTVAAEFPAY 960
TNYLYMTYNA DSHDLSFDDH GVMVLGSGVY RIGSSVEFDW CAVTAVRTLR ANNIKTIMVN 1020
YNPETVSTDY DEADRLYFET INLERVLDIY EIENSSGVVV SMGGQTSNNI AMTLHRENVK 1080
ILGTSPDMID SAENRYKFSR MLDQIGVDQP AWKELTSMDE AESFAEKVGY PVLVRPSYVL 1140
SGAAMNTVYS KNDLESYLNQ AVEVSRDYPV VITKYIENAK EIEMDAVARN GELVMHVVSE 1200
HVENAGVHSG DATLIVPPQD LAPETVDRIV VATAKIGKAL KITGPYNIQF IAKDNEIKVI 1260
ECNVRASRSF PFISKVVGVN LIELATKAIM GLPLTPYPVE KLPDDYVAVK VPQFSFPRLA 1320
GADPVLGVEM ASTGEVATFG HSKYEAYLKS LLATGFKLPK KNILLSIGSY KEKQELLSSV 1380
QKLYNMGYKL FATSGTADFL SEHGIAVQYL EVLNKDDDDQ KSEYSLTQHL ANNEIDLYIN 1440
LPSANRFRRP ASYVSKGYKT RRLAVDYSVP LVTNVKCAKL LIEAISRNIT LDVSERDAQT 1500
SHRTITLPGL INIATYVPNA SHVIKGPAEL KETTRLFLES GFTYCQLMPR SISGPVITDV 1560
ASLKAANSVS QDSSYTDFSF TIAGTAHNAH SVTQSASKVT ALFLPLRELK NKITAVAELL 1620
NQWPTEKQVI AEAKTADLAS VLLLTSLQNR SIHITGVSNK EDLALIMTVK AKDPRVTCDV 1680
NIYSLFIAQD DYPEAVFLPT KEDQEFFWNN LDSIDAFSVG ALPVALANVT GNKVDVGMGI 1740
KDSLPLLLAA VEEGKLTIDD IVLRLHDNPA KIFNIPTQDS VVEIDLDYSF RRNKRWSPFN 1800
KDMNGGIERV VYNGETLVLS GELVSPGAKG KCIVNPSPAS ITASAELQST SAKRRFSITE 1860
EAIADNLDAA EDAIPEQPLE QKLMSSRPPR ELVAPGAIQN LIRSNNPFRG RHILSIKQFK 1920
RSDFHVLFAV AQELRAAVAR EGVLDLMKGH VITTIFFEPS TRTCSSFIAA MERLGGRIVN 1980
VNPLVSSVKK GETLQDTIRT LACYSDAIVM RHSEEMSVHI AAKYSPVPII NGGNGSREHP 2040
TQAFLDLFTI REEIGTVNGI TVTFMGDLKH GRTVHSLCRL LMHYQVRINL VSPPELRLPE 2100
GLREELRKAG LLGVESIELT PHIISKTDVL YCTRVQEERF NSPEEYARLK DTYIVDNKIL 2160
AHAKENMAIM HPLPRVNEIK EEVDYDHRAA YFRQMKYGLF VRMALLAMVM GVDM 2214 
Gene Ontology
 GO:0016021; C:integral to membrane; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0016597; F:amino acid binding; IEA:InterPro.
 GO:0004070; F:aspartate carbamoyltransferase activity; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004088; F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IDA:SGD.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0070409; P:carbamoyl phosphate biosynthetic process; IEA:InterPro.
 GO:0006543; P:glutamine catabolic process; IEA:InterPro.
 GO:0006541; P:glutamine metabolic process; IDA:SGD.
 GO:0045984; P:negative regulation of pyrimidine nucleobase metabolic process; IDA:SGD. 
Interpro
 IPR006132; Asp/Orn_carbamoyltranf_P-bd.
 IPR006130; Asp/Orn_carbamoylTrfase.
 IPR002082; Asp_carbamoyltransf.
 IPR006131; Asp_carbamoyltransf_Asp/Orn-bd.
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR006275; CarbamoylP_synth_lsu.
 IPR005481; CarbamoylP_synth_lsu_N.
 IPR005480; CarbamoylP_synth_lsu_oligo.
 IPR006274; CarbamoylP_synth_ssu.
 IPR002474; CarbamoylP_synth_ssu_N.
 IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
 IPR005483; CbamoylP_synth_lsu_CPSase_dom.
 IPR017926; GATASE.
 IPR011607; MGS-like_dom.
 IPR016185; PreATP-grasp_dom. 
Pfam
 PF00289; CPSase_L_chain
 PF02786; CPSase_L_D2
 PF02787; CPSase_L_D3
 PF00988; CPSase_sm_chain
 PF00117; GATase
 PF02142; MGS
 PF00185; OTCace
 PF02729; OTCace_N 
SMART
 SM01096; CPSase_L_D3
 SM01097; CPSase_sm_chain
 SM00851; MGS 
PROSITE
 PS50975; ATP_GRASP
 PS00097; CARBAMOYLTRANSFERASE
 PS00866; CPSASE_1
 PS00867; CPSASE_2
 PS51273; GATASE_TYPE_1 
PRINTS
 PR00100; AOTCASE.
 PR00101; ATCASE.
 PR00098; CPSASE.