CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001367
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA (cytosine-5)-methyltransferase 3A 
Protein Synonyms/Alias
 Dnmt3a; DNA methyltransferase MmuIIIA; DNA MTase MmuIIIA; M.MmuIIIA 
Gene Name
 Dnmt3a 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
196GDPYYISKRKRDEWLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Can actively repress transcription through the recruitment of HDAC activity. 
Sequence Annotation
 DOMAIN 288 346 PWWP.
 DOMAIN 478 610 ADD.
 ZN_FING 489 519 GATA-type; atypical.
 ZN_FING 530 586 PHD-type; atypical.
 REGION 195 399 Interaction with DNMT1 and DNMT3B (By
 REGION 490 582 Interaction with the PRC2/EED-EZH2
 REGION 637 641 S-adenosyl-L-methionine binding (By
 REGION 682 684 S-adenosyl-L-methionine binding (By
 REGION 887 889 S-adenosyl-L-methionine binding (By
 ACT_SITE 706 706 By similarity.
 BINDING 660 660 S-adenosyl-L-methionine (By similarity).
 MOD_RES 102 102 Phosphoserine (By similarity).
 MOD_RES 239 239 Phosphoserine (By similarity).
 MOD_RES 251 251 Phosphoserine (By similarity).
 MOD_RES 257 257 Phosphothreonine (By similarity).  
Keyword
 3D-structure; Alternative promoter usage; Chromatin regulator; Complete proteome; Cytoplasm; DNA-binding; Metal-binding; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Repressor; S-adenosyl-L-methionine; Transcription; Transcription regulation; Transferase; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 908 AA 
Protein Sequence
MPSSGPGDTS SSSLEREDDR KEGEEQEENR GKEERQEPSA TARKVGRPGR KRKHPPVESS 60
DTPKDPAVTT KSQPMAQDSG PSDLLPNGDL EKRSEPQPEE GSPAAGQKGG APAEGEGTET 120
PPEASRAVEN GCCVTKEGRG ASAGEGKEQK QTNIESMKME GSRGRLRGGL GWESSLRQRP 180
MPRLTFQAGD PYYISKRKRD EWLARWKREA EKKAKVIAVM NAVEENQASG ESQKVEEASP 240
PAVQQPTDPA SPTVATTPEP VGGDAGDKNA TKAADDEPEY EDGRGFGIGE LVWGKLRGFS 300
WWPGRIVSWW MTGRSRAAEG TRWVMWFGDG KFSVVCVEKL MPLSSFCSAF HQATYNKQPM 360
YRKAIYEVLQ VASSRAGKLF PACHDSDESD SGKAVEVQNK QMIEWALGGF QPSGPKGLEP 420
PEEEKNPYKE VYTDMWVEPE AAAYAPPPPA KKPRKSTTEK PKVKEIIDER TRERLVYEVR 480
QKCRNIEDIC ISCGSLNVTL EHPLFIGGMC QNCKNCFLEC AYQYDDDGYQ SYCTICCGGR 540
EVLMCGNNNC CRCFCVECVD LLVGPGAAQA AIKEDPWNCY MCGHKGTYGL LRRREDWPSR 600
LQMFFANNHD QEFDPPKVYP PVPAEKRKPI RVLSLFDGIA TGLLVLKDLG IQVDRYIASE 660
VCEDSITVGM VRHQGKIMYV GDVRSVTQKH IQEWGPFDLV IGGSPCNDLS IVNPARKGLY 720
EGTGRLFFEF YRLLHDARPK EGDDRPFFWL FENVVAMGVS DKRDISRFLE SNPVMIDAKE 780
VSAAHRARYF WGNLPGMNRP LASTVNDKLE LQECLEHGRI AKFSKVRTIT TRSNSIKQGK 840
DQHFPVFMNE KEDILWCTEM ERVFGFPVHY TDVSNMSRLA RQRLLGRSWS VPVIRHLFAP 900
LKEYFACV 908 
Gene Ontology
 GO:0000775; C:chromosome, centromeric region; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0000791; C:euchromatin; ISS:UniProtKB.
 GO:0005720; C:nuclear heterochromatin; IDA:MGI.
 GO:0016363; C:nuclear matrix; ISS:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; ISS:UniProtKB.
 GO:0051718; F:DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; IEA:Compara.
 GO:0003677; F:DNA binding; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0045322; F:unmethylated CpG binding; IEA:Compara.
 GO:0071230; P:cellular response to amino acid stimulus; IDA:MGI.
 GO:0043045; P:DNA methylation involved in embryo development; IMP:UniProtKB.
 GO:0043046; P:DNA methylation involved in gamete generation; IMP:UniProtKB.
 GO:0010424; P:DNA methylation on cytosine within a CG sequence; IEA:Compara.
 GO:0044027; P:hypermethylation of CpG island; IDA:BHF-UCL.
 GO:0010216; P:maintenance of DNA methylation; TAS:BHF-UCL.
 GO:0006346; P:methylation-dependent chromatin silencing; IDA:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0006349; P:regulation of gene expression by genetic imprinting; IDA:BHF-UCL.
 GO:0046498; P:S-adenosylhomocysteine metabolic process; IEA:Compara.
 GO:0046499; P:S-adenosylmethioninamine metabolic process; IEA:Compara.
 GO:0007283; P:spermatogenesis; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR025766; ADD.
 IPR018117; C5_DNA_meth_AS.
 IPR001525; C5_MeTfrase.
 IPR025811; C5_MeTrfase_3.
 IPR000313; PWWP.
 IPR011011; Znf_FYVE_PHD. 
Pfam
 PF00145; DNA_methylase
 PF00855; PWWP 
SMART
 SM00293; PWWP 
PROSITE
 PS51533; ADD
 PS00094; C5_MTASE_1
 PS00095; C5_MTASE_2
 PS50812; PWWP 
PRINTS