CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012809
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inverted formin-2 
Protein Synonyms/Alias
 HBEBP2-binding protein C 
Gene Name
 INF2 
Gene Synonyms/Alias
 C14orf151; C14orf173 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MSVKEGAQRKWubiquitination[1]
15QRKWAALKEKLGPQDubiquitination[1]
17KWAALKEKLGPQDSDubiquitination[1]
140DTSNVMVKKQVFELLubiquitination[2]
565NPPTLRMKKLNWQKLubiquitination[3]
566PPTLRMKKLNWQKLPubiquitination[3]
571MKKLNWQKLPSNVARubiquitination[1, 3]
612LFSFPAAKPKEPTMVubiquitination[2, 3]
673KFDVEVLKQLLKLLPubiquitination[3]
682LLKLLPEKHEIENLRubiquitination[3]
846EASSNLKKLLETERKubiquitination[1]
889LFEAIEQKQRELADYubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Severs actin filaments and accelerates their polymerization and depolymerization (By similarity). 
Sequence Annotation
 DOMAIN 1 330 GBD/FH3.
 DOMAIN 554 946 FH2.
 DOMAIN 974 989 WH2.
 MOD_RES 1147 1147 Phosphoserine.
 MOD_RES 1149 1149 Phosphoserine.
 MOD_RES 1179 1179 Phosphothreonine.
 MOD_RES 1192 1192 Phosphoserine.
 MOD_RES 1206 1206 Phosphothreonine.  
Keyword
 Actin-binding; Alternative splicing; Charcot-Marie-Tooth disease; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Neuropathy; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1249 AA 
Protein Sequence
MSVKEGAQRK WAALKEKLGP QDSDPTEANL ESADPELCIR LLQMPSVVNY SGLRKRLEGS 60
DGGWMVQFLE QSGLDLLLEA LARLSGRGVA RISDALLQLT CVSCVRAVMN SRQGIEYILS 120
NQGYVRQLSQ ALDTSNVMVK KQVFELLAAL CIYSPEGHVL TLDALDHYKT VCSQQYRFSI 180
VMNELSGSDN VPYVVTLLSV INAVILGPED LRARTQLRNE FIGLQLLDVL ARLRDLEDAD 240
LLIQLEAFEE AKAEDEEELL RVSGGVDMSS HQEVFASLFH KVSCSPVSAQ LLSVLQGLLH 300
LEPTLRSSQL LWEALESLVN RAVLLASDAQ ECTLEEVVER LLSVKGRPRP SPLVKAHKSV 360
QANLDQSQRG SSPQNTTTPK PSVEGQQPAA AAACEPVDHA QSESILKVSQ PRALEQQAST 420
PPPPPPPPLL PGSSAEPPPP PPPPPLPSVG AKALPTAPPP PPLPGLGAMA PPAPPLPPPL 480
PGSCEFLPPP PPPLPGLGCP PPPPPLLPGM GWGPPPPPPP LLPCTCSPPV AGGMEEVIVA 540
QVDHGLGSAW VPSHRRVNPP TLRMKKLNWQ KLPSNVAREH NSMWASLSSP DAEAVEPDFS 600
SIERLFSFPA AKPKEPTMVA PRARKEPKEI TFLDAKKSLN LNIFLKQFKC SNEEVAAMIR 660
AGDTTKFDVE VLKQLLKLLP EKHEIENLRA FTEERAKLAS ADHFYLLLLA IPCYQLRIEC 720
MLLCEGAAAV LDMVRPKAQL VLAACESLLT SRQLPIFCQL ILRIGNFLNY GSHTGDADGF 780
KISTLLKLTE TKSQQNRVTL LHHVLEEAEK SHPDLLQLPR DLEQPSQAAG INLEIIRSEA 840
SSNLKKLLET ERKVSASVAE VQEQYTERLQ ASISAFRALD ELFEAIEQKQ RELADYLCED 900
AQQLSLEDTF STMKAFRDLF LRALKENKDR KEQAAKAERR KQQLAEEEAR RPRGEDGKPV 960
RKGPGKQEEV CVIDALLADI RKGFQLRKTA RGRGDTDGGS KAASMDPPRA TEPVATSNPA 1020
GDPVGSTRCP ASEPGLDATT ASESRGWDLV DAVTPGPQPT LEQLEEGGPR PLERRSSWYV 1080
DASDVLTTED PQCPQPLEGA WPVTLGDAQA LKPLKFSSNQ PPAAGSSRQD AKDPTSLLGV 1140
LQAEADSTSE GLEDAVHSRG ARPPAAGPGG DEDEDEEDTA PESALDTSLD KSFSEDAVTD 1200
SSGSGTLPRA RGRASKGTGK RRKKRPSRSQ EEVPPDSDDN KTKKLCVIQ 1249 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
 GO:0090140; P:regulation of mitochondrial fission; IMP:MGI. 
Interpro
 IPR003104; Actin-bd_FH2/DRF_autoreg.
 IPR016024; ARM-type_fold.
 IPR010472; Drf_FH3.
 IPR010473; Drf_GTPase-bd.
 IPR015425; FH2_actin-bd.
 IPR014768; GTPase-bd/formin_homology_3.
 IPR003124; WH2_dom. 
Pfam
 PF06367; Drf_FH3
 PF06371; Drf_GBD
 PF02181; FH2
 PF02205; WH2 
SMART
 SM00498; FH2
 SM00246; WH2 
PROSITE
 PS51444; FH2
 PS51232; GBD_FH3
 PS51082; WH2 
PRINTS