CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023572
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Membrane steroid-binding protein 1 
Protein Synonyms/Alias
 AtMP1 
Gene Name
 MSBP1 
Gene Synonyms/Alias
 MP1; At5g52240; F17P19.14 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
122PYALFAGKDASRALAubiquitination[1]
130DASRALAKMSFEEKDubiquitination[1]
166KFMSKYAKVGTVKVAubiquitination[1]
217APAETVLKKEE****ubiquitination[1]
218PAETVLKKEE*****ubiquitination[1]
Reference
 [1] E3-independent ubiquitination of AtMAPR/MSBP1.
 Kao AL, Lin YH, Chen RP, Huang YY, Chen CC, Yang CC.
 Phytochemistry. 2012 Jun;78:7-19. [PMID: 22513011] 
Functional Description
 MSBP1 can bind to multiple steroid compounds with different affinities. Negatively regulates cell elongation and brassinosteroid signaling. May act as a coreceptor with BAK1 and enhances its endocytosis. 
Sequence Annotation
 DOMAIN 74 171 Cytochrome b5 heme-binding.
 REGION 74 171 Steroid-binding (By similarity).
 METAL 110 110 Iron (heme axial ligand) (By similarity).
 METAL 134 134 Iron (heme axial ligand) (By similarity).  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Endosome; Iron; Lipid-binding; Membrane; Metal-binding; Reference proteome; Signal-anchor; Steroid-binding; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 220 AA 
Protein Sequence
MALELWQTLK EAIHAYTGLS PVVFFTALAL AFAIYQVISG WFASPFDDVN RHQRARSLAQ 60
EEEPPIPQPV QVGEITEEEL KQYDGSDPQK PLLMAIKHQI YDVTQSRMFY GPGGPYALFA 120
GKDASRALAK MSFEEKDLTW DVSGLGPFEL DALQDWEYKF MSKYAKVGTV KVAGSEPETA 180
SVSEPTENVE QDAHVTTTPG KTVVDKSDDA PAETVLKKEE 220 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:TAIR.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:TAIR.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005496; F:steroid binding; IDA:TAIR.
 GO:0030308; P:negative regulation of cell growth; IMP:TAIR. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd. 
Pfam
 PF00173; Cyt-b5 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS