CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004609
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peptidyl-prolyl cis-trans isomerase A 
Protein Synonyms/Alias
 PPIase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A; SP18; Peptidyl-prolyl cis-trans isomerase A, N-terminally processed 
Gene Name
 Ppia 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
28SFELFADKVPKTAENacetylation[1, 2, 3, 4]
28SFELFADKVPKTAENsuccinylation[4]
28SFELFADKVPKTAENubiquitination[5]
31LFADKVPKTAENFRAubiquitination[5]
44RALSTGEKGFGYKGSubiquitination[5]
49GEKGFGYKGSSFHRIubiquitination[5]
82GRSIYGEKFEDENFIacetylation[3]
82GRSIYGEKFEDENFIubiquitination[5]
125KTEWLDGKHVVFGKVacetylation[3]
125KTEWLDGKHVVFGKVubiquitination[5]
131GKHVVFGKVKEGMNIacetylation[2, 3]
131GKHVVFGKVKEGMNIubiquitination[5]
133HVVFGKVKEGMNIVEacetylation[4, 6]
133HVVFGKVKEGMNIVEubiquitination[5]
155RNGKTSKKITISDCGacetylation[6]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441
Functional Description
 PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. 
Sequence Annotation
 DOMAIN 7 163 PPIase cyclophilin-type.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylvaline; in Peptidyl-prolyl cis-
 MOD_RES 21 21 Phosphoserine.
 MOD_RES 28 28 N6-acetyllysine; alternate (By
 MOD_RES 44 44 N6-acetyllysine (By similarity).
 MOD_RES 82 82 N6-acetyllysine (By similarity).
 MOD_RES 93 93 Phosphothreonine (By similarity).
 MOD_RES 125 125 N6-acetyllysine (By similarity).
 MOD_RES 131 131 N6-acetyllysine (By similarity).
 CARBOHYD 108 108 N-linked (GlcNAc...) (Potential).
 CROSSLNK 28 28 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Complete proteome; Cyclosporin; Cytoplasm; Direct protein sequencing; Glycoprotein; Isomerase; Isopeptide bond; Phosphoprotein; Reference proteome; Rotamase; Secreted; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 164 AA 
Protein Sequence
MVNPTVFFDI TADDEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SSFHRIIPGF 60
MCQGGDFTRH NGTGGRSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE 120
WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITISD CGQL 164 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0042277; F:peptide binding; IEA:UniProtKB-KW.
 GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; ISS:UniProtKB.
 GO:0006457; P:protein folding; IEA:UniProtKB-KW.
 GO:0045069; P:regulation of viral genome replication; ISS:UniProtKB. 
Interpro
 IPR002130; Cyclophilin-like_PPIase_dom.
 IPR024936; Cyclophilin-type_PPIase.
 IPR020892; Cyclophilin-type_PPIase_CS. 
Pfam
 PF00160; Pro_isomerase 
SMART
  
PROSITE
 PS00170; CSA_PPIASE_1
 PS50072; CSA_PPIASE_2 
PRINTS
 PR00153; CSAPPISMRASE.