CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041339
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HCG2044781 
Protein Synonyms/Alias
 Protein TMEM189-UBE2V1; Transmembrane protein 189 
Gene Name
 TMEM189 
Gene Synonyms/Alias
 TMEM189-UBE2V1; hCG_2044781 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
291ENRIYSLKIECGPKYubiquitination[1, 2, 3]
297LKIECGPKYPEAPPFacetylation[3, 4]
297LKIECGPKYPEAPPFubiquitination[2, 3, 5]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 370 AA 
Protein Sequence
MAGAENWPGQ QLELDEDEAS CCRWGAQHAG ARELAALYSP GKRLQEWCSV ILCFSLIAHN 60
LVHLLLLARW EDTPLVILGV VAGALIADFL SGLVHWGADT WGSVELPIVG KAFIRPFREH 120
HIDPTAITRH DFIETNGDNC LVTLLPLLNM AYKFRTHSPE ALEQLYPWEC FVFCLIIFGT 180
FTNQIHKWSH TYFGLPRWVT LLQDWHVILP RKHHRIHHVS PHETYFCITT GVKVPRNFRL 240
LEELEEGQKG VGDGTVSWGL EDDEDMTLTR WTGMIIGPPR TIYENRIYSL KIECGPKYPE 300
APPFVRFVTK INMNGVNSSN GVVDPRAISV LAKWQNSYSI KVVLQELRRL MMSKENMKLP 360
QPPEGQCYSN 370 
Gene Ontology
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro. 
Interpro
 IPR019547; KuaUb-conj-enz_UEV1.
 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF10520; Kua-UEV1_localn
 PF00179; UQ_con 
SMART
  
PROSITE
 PS50127; UBIQUITIN_CONJUGAT_2 
PRINTS