| Tag | Content |
|---|
| CPLM ID | CPLM-024221 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Phosphoenolpyruvate carboxykinase [GTP] |
Protein Synonyms/Alias | PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase |
Gene Name | pckG |
Gene Synonyms/Alias | MSMEG_0255; MSMEI_0248 |
Created Date | July 27, 2013 |
Organism | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) |
NCBI Taxa ID | 246196 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 485 | AHWINVGKNADESKL | pupylation | [1] |
|
Reference | [1] Expansion of the mycobacterial "PUPylome". Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC. Mol Biosyst. 2010 Feb;6(2):376-85. [ PMID: 20094657] |
Functional Description | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity). |
Sequence Annotation | NP_BIND 272 277 GTP (By similarity).
NP_BIND 514 517 GTP (By similarity).
REGION 386 388 Substrate binding (By similarity).
ACT_SITE 273 273 By similarity.
METAL 229 229 Manganese (By similarity).
METAL 249 249 Manganese (By similarity).
METAL 296 296 Manganese (By similarity).
BINDING 81 81 Substrate (By similarity).
BINDING 222 222 Substrate; via amide nitrogen (By
BINDING 229 229 Substrate (By similarity).
BINDING 271 271 Substrate (By similarity).
BINDING 388 388 GTP (By similarity).
BINDING 419 419 GTP (By similarity).
CROSSLNK 485 485 Isoglutamyl lysine isopeptide (Lys-Gln) |
Keyword | Complete proteome; Cytoplasm; Decarboxylase; Gluconeogenesis; GTP-binding; Isopeptide bond; Lyase; Manganese; Metal-binding; Nucleotide-binding; Reference proteome; Ubl conjugation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 608 AA |
Protein Sequence | MTSATIPGLD TAPTKHQGLL AWVQEVAELT QPDRVVFADG SDEEYERLCA HLVEAGTFQK 60
LNPEKQPNSY LALSDPSDVA RVESRTFICT EREIDAGPTN NWMDPAEMRG IMTDLYRGSM 120
RGRTLYVVPF CMGPLDAEDP KLGVEITDSE YVVVSMRTMT RMGRAALDKL GDDGFFVKAL 180
HSIGAPLEPG QKDVPWPCND TKYITHFPET REIWSFGSGY GGNALLGKKC YSLRIASAMA 240
HDEGWLAEHM LILKLISPEN KAYFIAAAFP SACGKTNLAM LQPTIEGWRA ETVGDDIAWM 300
RFGKDGRLYA TNPEFGFFGV APGTNWSSNP NAMKTIAAGN TVFTNVAKTD DGDVWWEGLE 360
GDPQHLIDWK GNDWTPESGE KAAHPNSRYC TPISQCPTLA PEWDDPQGVP ISAILFGGRR 420
KTTVPLITEA RDWQHGVFIG ATLGSEQTAA AEGKVGTVRR DPMAMLPFLG YNVGDYFAHW 480
INVGKNADES KLPKVFFVNW FRRGDDGRFL WPGFGENSRV LKWAVERIEH KADGKSTPIG 540
IVPTAADLDL EGLDVDPADV DEALAVKPEE WRAELPLIEE WFEFVGEKLP TGLKDEFDAL 600
KHRLSEEG 608 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0005525; F:GTP binding; IEA:HAMAP. GO:0030145; F:manganese ion binding; IEA:HAMAP. GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:HAMAP. GO:0006094; P:gluconeogenesis; IEA:HAMAP. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |