UniProt Accession

 3BHS3_MOUSE; P26150 

Genbank Protein ID

 M77015 

Genbank Nucleotide ID

  

Protein Name

 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 3 

Protein Synonyms/Alias

 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type III; 3-beta-HSD III; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase 

Gene Name

 Hsd3b3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
37	EEIRVLDKVFKPETR	acetylation	[1]
37	EEIRVLDKVFKPETR	ubiquitination	[2]
159	SDPYPYSKKMAEKAV	ubiquitination	[2]
164	YSKKMAEKAVLAANG	ubiquitination	[2]
175	AANGSMLKNGGTLQT	acetylation	[1]
204	FLSNTIIKALKNKFI	ubiquitination	[2]
245	RGLRNPKKSPNIQGE	ubiquitination	[2]
350	LVSWEEAKQKTSEWI	ubiquitination	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. 

Sequence Annotation

 ACT_SITE 155 155 Proton acceptor (By similarity).
 BINDING 159 159 NAD (By similarity).  

Keyword

 Complete proteome; Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion; Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome; Steroidogenesis; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 373 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:EC.
 GO:0004769; F:steroid delta-isomerase activity; IEA:EC.
 GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR002225; 3Beta_OHSteriod_DH/Estase.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF01073; 3Beta_HSD 

SMART

  

PROSITE

  

PRINTS