UniProt Accession

 NUP50_MOUSE; Q9JIH2; Q3TG43; Q3TN17; Q6P1F4; Q6PAL4; Q8C2Y6; Q9CU02; Q9JK85 

Genbank Protein ID

 AF229256; AF251799; AK018983; AK087706; AK133371; AK133562; AK141750; AK151947; AK152309; AK161713; AK165581; AK168888; CH466550; BC059239; BC060234; BC065102 

Genbank Nucleotide ID

 AAF34721.1; AAF70057.1; BAB31500.1; BAC39975.1; BAE21620.1; BAE21727.1; BAE24822.1; BAE30821.1; BAE31115.1; BAE36546.1; BAE38272.1; BAE40705.1; EDL04446.1; AAH59239.1; AAH60234.1; AAH65102.1 

Protein Name

 Nuclear pore complex protein Nup50 

Protein Synonyms/Alias

 50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50 

Gene Name

 Nup50 

Gene Synonyms/Alias

 Npap60 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
8	MAKRVAEKELTDRNW	acetylation	[1, 2]
59	SDSGGAFKGFKGLVV	acetylation	[1]
59	SDSGGAFKGFKGLVV	ubiquitination	[3]
126	GPTTLVDKKISSPKC	acetylation	[2, 4]
172	SVRDWIVKHVNTNPL	acetylation	[1]
229	PSLFGSTKLQQESPF	ubiquitination	[3]
257	VEFTAEKKSDAAQGA	acetylation	[1]
273	SASFSFGKKIESSAL	acetylation	[1]
273	SASFSFGKKIESSAL	ubiquitination	[3]
448	ATMLIRVKTSEDADE	acetylation	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. 

Sequence Annotation

 REPEAT 76 77 1.
 REPEAT 112 113 2.
 REPEAT 225 226 3.
 REPEAT 271 272 4.
 REPEAT 301 302 5.
 DOMAIN 333 466 RanBD1.
 REGION 76 302 5 X 2 AA repeats of F-G.
 REGION 143 205 Binding to CDKN1B.
 MOD_RES 82 82 N6-acetyllysine (By similarity).
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 246 246 Phosphothreonine (By similarity).
 MOD_RES 294 294 Phosphoserine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 466 AA 

Protein Sequence

Gene Ontology

 GO:0031965; C:nuclear membrane; ISS:UniProtKB.
 GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
 GO:0046907; P:intracellular transport; IEA:InterPro.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR015007; NUP2/50/61.
 IPR011993; PH_like_dom.
 IPR000156; Ran_bind_dom. 

Pfam

 PF08911; NUP50
 PF00638; Ran_BP1 

SMART

 SM00160; RanBD 

PROSITE

 PS50196; RANBD1 

PRINTS