| Tag | Content |
|---|
| CPLM ID | CPLM-003470 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB |
Protein Synonyms/Alias | tRNA-i(6)A37 methylthiotransferase |
Gene Name | miaB |
Gene Synonyms/Alias | yleA; b0661; JW0658 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 58 | IREKAQEKVFHQLGR | acetylation | [1] | | 287 | THTALEYKAIIRKLR | acetylation | [1] | | 320 | ETTEDFEKTMKLIAD | acetylation | [1] | | 395 | LVEGTSRKSIMELSG | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. |
Sequence Annotation | DOMAIN 3 120 MTTase N-terminal.
DOMAIN 378 441 TRAM.
METAL 12 12 Iron-sulfur (4Fe-4S) (By similarity).
METAL 49 49 Iron-sulfur (4Fe-4S) (By similarity).
METAL 83 83 Iron-sulfur (4Fe-4S) (By similarity).
METAL 157 157 Iron-sulfur (4Fe-4S-S-AdoMet) (Probable).
METAL 161 161 Iron-sulfur (4Fe-4S-S-AdoMet) (Probable).
METAL 164 164 Iron-sulfur (4Fe-4S-S-AdoMet) (Probable). |
Keyword | 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur; Metal-binding; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 474 AA |
Protein Sequence | MTKKLHIKTW GCQMNEYDSS KMADLLDATH GYQLTDVAEE ADVLLLNTCS IREKAQEKVF 60
HQLGRWKLLK EKNPDLIIGV GGCVASQEGE HIRQRAHYVD IIFGPQTLHR LPEMINSVRG 120
DRSPVVDISF PEIEKFDRLP EPRAEGPTAF VSIMEGCNKY CTYCVVPYTR GEEVSRPSDD 180
ILFEIAQLAA QGVREVNLLG QNVNAWRGEN YDGTTGSFAD LLRLVAAIDG IDRIRFTTSH 240
PIEFTDDIIE VYRDTPELVS FLHLPVQSGS DRILNLMGRT HTALEYKAII RKLRAARPDI 300
QISSDFIVGF PGETTEDFEK TMKLIADVNF DMSYSFIFSA RPGTPAADMV DDVPEEEKKQ 360
RLYILQERIN QQAMAWSRRM LGTTQRILVE GTSRKSIMEL SGRTENNRVV NFEGTPDMIG 420
KFVDVEITDV YPNSLRGKVV RTEDEMGLRV AETPESVIAR TRKENDLGVG YYQP 474 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |