CPLM-001099

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-001099

UniProt Accession

PLCH2_HUMAN; O75038; A2VCM3; B9DI80; Q3LUA8; Q86XJ2; Q86XU1; Q86YU7; Q8TEH5; Q8WUS6

Genbank Protein ID

DQ176850; AK074149; AK122591; AB007919; AL139246; BC019679; BC043358; BC050037; BC128207

Genbank Nucleotide ID

ABA12209.1; BAB84975.1; BAC56932.2; BAA32295.3; CAX30815.1; AAH19679.1; AAH43358.1; AAH50037.1; AAI28208.1

Protein Name

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2

Protein Synonyms/Alias

Phosphoinositide phospholipase C-eta-2; Phosphoinositide phospholipase C-like 4; PLC-L4; Phospholipase C-like protein 4; Phospholipase C-eta-2; PLC-eta2

Gene Name

PLCH2

Gene Synonyms/Alias

KIAA0450; PLCL4

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
621	TRQKKTMKLSRALSD	ubiquitination	[1]

Reference

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain (By similarity).

Sequence Annotation

DOMAIN 47 155 PH.
DOMAIN 169 204 EF-hand 1.
DOMAIN 205 241 EF-hand 2.
DOMAIN 326 471 PI-PLC X-box.
DOMAIN 626 740 PI-PLC Y-box.
DOMAIN 745 852 C2.
REGION 1 155 Necessary for plasma membrane
ACT_SITE 341 341 By similarity.
ACT_SITE 385 385 By similarity.
METAL 342 342 Calcium 1; catalytic (By similarity).
METAL 371 371 Calcium 1; catalytic (By similarity).
METAL 373 373 Calcium 1; catalytic (By similarity).
METAL 420 420 Calcium 1; catalytic (By similarity).
METAL 784 784 Calcium 2; via carbonyl oxygen (By
METAL 786 786 Calcium 2 (By similarity).
METAL 810 810 Calcium 2 (By similarity).
METAL 839 839 Calcium 3 (By similarity).
METAL 840 840 Calcium 3; via carbonyl oxygen (By
METAL 841 841 Calcium 3 (By similarity).
BINDING 469 469 Substrate (By similarity).
BINDING 471 471 Substrate (By similarity).
BINDING 653 653 Substrate (By similarity).
BINDING 680 680 Substrate (By similarity).
MOD_RES 595 595 Phosphoserine (By similarity).
MOD_RES 605 605 Phosphoserine (By similarity).

Keyword

Alternative splicing; Calcium; Cell membrane; Complete proteome; Cytoplasm; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Transducer.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1416 AA

Protein Sequence

MSGPWPSPDS RTKGTVAWLA EVLLWVGGSV VLSSEWQLGP LVERCMGAMQ EGMQMVKLRG 60
GSKGLVRFYY LDEHRSCIRW RPSRKNEKAK ISIDSIQEVS EGRQSEVFQR YPDGSFDPNC 120
CFSIYHGSHR ESLDLVSTSS EVARTWVTGL RYLMAGISDE DSLARRQRTR DQWLKQTFDE 180
ADKNGDGSLS IGEVLQLLHK LNVNLPRQRV KQMFREADTD DHQGTLGFEE FCAFYKMMST 240
RRDLYLLMLT YSNHKDHLDA ASLQRFLQVE QKMAGVTLES CQDIIEQFEP CPENKSKGLL 300
GIDGFTNYTR SPAGDIFNPE HHHVHQDMTQ PLSHYFITSS HNTYLVGDQL MSQSRVDMYA 360
WVLQAGCRCV EVDCWDGPDG EPIVHHGYTL TSKILFKDVI ETINKYAFIK NEYPVILSIE 420
NHCSVIQQKK MAQYLTDILG DKLDLSSVSS EDATTLPSPQ MLKGKILVKG KKLPANISED 480
AEEGEVSDED SADEIDDDCK LLNGDASTNR KRVENTAKRK LDSLIKESKI RDCEDPNNFS 540
VSTLSPSGKL GRKSKAEEDV ESGEDAGASR RNGRLVVGSF SRRKKKGSKL KKAASVEEGD 600
EGQDSPGGQS RGATRQKKTM KLSRALSDLV KYTKSVATHD IEMEAASSWQ VSSFSETKAH 660
QILQQKPAQY LRFNQQQLSR IYPSSYRVDS SNYNPQPFWN AGCQMVALNY QSEGRMLQLN 720
RAKFSANGGC GYVLKPGCMC QGVFNPNSED PLPGQLKKQL VLRIISGQQL PKPRDSMLGD 780
RGEIIDPFVE VEIIGLPVDC SREQTRVVDD NGFNPTWEET LVFMVHMPEI ALVRFLVWDH 840
DPIGRDFIGQ RTLAFSSMMP GYRHVYLEGM EEASIFVHVA VSDISGKVKQ ALGLKGLFLR 900
GPKPGSLDSH AAGRPPARPS VSQRILRRTA SAPTKSQKPG RRGFPELVLG TRDTGSKGVA 960
DDVVPPGPGP APEAPAQEGP GSGSPRDTRP LSTQRPLPPL CSLETIAEEP APGPGPPPPA 1020
AVPTSSSQGR PPYPTGPGAN VASPLEDTEE PRDSRPRPCN GEGAGGAYER APGSQTDGRS 1080
QPRTLGHLPV IRRVKSEGQV PTEPLGGWRP LAAPFPAPAV YSDATGSDPL WQRLEPCGHR 1140
DSVSSSSSMS SSDTVIDLSL PSLGLGRSRE NLAGAHMGRL PPRPHSASAA RPDLPPVTKS 1200
KSNPNLRATG QRPPIPDELQ PRSLAPRMAG LPFRPPWGCL SLVGVQDCPV AAKSKSLGDL 1260
TADDFAPSFE GGSRRLSHSL GLPGGTRRVS GPGVRRDTLT EQLRWLTVFQ QAGDITSPTS 1320
LGPAGEGVAG GPGFVRRSSS RSHSRVRAIA SRARQAQERQ QRLQGLGRQG PPEEERGTPE 1380
GACSVGHEGS VDAPAPSKGA LGPASAAAEN LVLLRL 1416

Gene Ontology

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0005509; F:calcium ion binding; IEA:InterPro.
GO:0004435; F:phosphatidylinositol phospholipase C activity; IEA:EC.
GO:0005543; F:phospholipid binding; IEA:InterPro.
GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
GO:0043647; P:inositol phosphate metabolic process; TAS:Reactome.
GO:0035556; P:intracellular signal transduction; IEA:InterPro.
GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
GO:0046488; P:phosphatidylinositol metabolic process; IEA:Compara.

Interpro

IPR000008; C2_Ca-dep.
IPR008973; C2_Ca/lipid-bd_dom_CaLB.
IPR018029; C2_membr_targeting.
IPR011992; EF-hand-like_dom.
IPR018247; EF_Hand_1_Ca_BS.
IPR002048; EF_hand_dom.
IPR011993; PH_like_dom.
IPR001192; Pinositol_PLipase_C.
IPR017946; PLC-like_Pdiesterase_TIM-brl.
IPR001849; Pleckstrin_homology.
IPR015359; PLipase_C_EF-hand-like.
IPR000909; PLipase_C_PInositol-sp_X_dom.
IPR001711; PLipase_C_Pinositol-sp_Y.

Pfam

PF00168; C2
PF13499; EF_hand_5
PF09279; efhand_like
PF00388; PI-PLC-X
PF00387; PI-PLC-Y

SMART

SM00239; C2
SM00054; EFh
SM00233; PH
SM00148; PLCXc
SM00149; PLCYc

PROSITE

PS50004; C2
PS00018; EF_HAND_1
PS50222; EF_HAND_2
PS50003; PH_DOMAIN
PS50007; PIPLC_X_DOMAIN
PS50008; PIPLC_Y_DOMAIN

PRINTS

PR00390; PHPHLIPASEC.