CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022678
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein quaking 
Protein Synonyms/Alias
 MqkI; qkI 
Gene Name
 Qki 
Gene Synonyms/Alias
 Qk; Qk1; Qka1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
54EEISRVRKDMYNDTLubiquitination[1]
91EKLYVPVKEYPDFNFubiquitination[1]
190AEGEDSLKKMQLMELubiquitination[1]
210TYRDANIKSPALAFSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 RNA-binding protein that plays a central role in myelinization. Also required for visceral endoderm function and blood vessel development. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation, as well as cellular processes including apoptosis, cell cycle, glial cell fate and development. Required to protect and promote stability of mRNAs such as MBP and CDKN1B which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Isoform 1 is involved in regulation of mRNA splicing of MAG pre-mRNA by acting as a negative regulator of MAG exon 12 alternative splicing. Isoform 3 can induce apoptosis, while heterodimerization with other isoforms results in nuclear translocation of isoform 3 and suppression of apoptosis. Isoform 4 acts as a translational repressor for GLI1. May also play a role in smooth muscle development. 
Sequence Annotation
 DOMAIN 87 153 KH.
 MOTIF 276 279 SH3-binding.
 MOTIF 324 330 Nuclear localization signal.
 MOD_RES 242 242 Asymmetric dimethylarginine; by CARM1 (By  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Methylation; mRNA processing; mRNA splicing; mRNA transport; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; SH3-binding; Translation regulation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 341 AA 
Protein Sequence
MVGEMETKEK PKPTPDYLMQ LMNDKKLMSS LPNFCGIFNH LERLLDEEIS RVRKDMYNDT 60
LNGSTEKRSA ELPDAVGPIV QLQEKLYVPV KEYPDFNFVG RILGPRGLTA KQLEAETGCK 120
IMVRGKGSMR DKKKEEQNRG KPNWEHLNED LHVLITVEDA QNRAEIKLKR AVEEVKKLLV 180
PAAEGEDSLK KMQLMELAIL NGTYRDANIK SPALAFSLAA TAQAAPRIIT GPAPVLPPAA 240
LRTPTPAGPT IMPLIRQIQT AVMPNGTPHP TAAIVPPGPE AGLIYTPYEY PYTLAPATSI 300
LEYPIEPSGV LGAVATKVRR HDMRVHPYQR IVTADRAATG NVNW 344 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:MGI.
 GO:0005634; C:nucleus; TAS:MGI.
 GO:0003723; F:RNA binding; TAS:MGI.
 GO:0042759; P:long-chain fatty acid biosynthetic process; IMP:MGI.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0042692; P:muscle cell differentiation; IMP:MGI.
 GO:0042552; P:myelination; IMP:MGI.
 GO:0010628; P:positive regulation of gene expression; IMP:MGI.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0007286; P:spermatid development; IGI:MGI.
 GO:0001570; P:vasculogenesis; IMP:MGI. 
Interpro
 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1. 
Pfam
 PF00013; KH_1 
SMART
 SM00322; KH 
PROSITE
 PS50084; KH_TYPE_1 
PRINTS