CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012661
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasomal ubiquitin receptor ADRM1 
Protein Synonyms/Alias
 110 kDa cell membrane glycoprotein; Gp110; Adhesion-regulating molecule 1; ARM-1; Proteasome regulatory particle non-ATPase 13; hRpn13; Rpn13 homolog 
Gene Name
 ADRM1 
Gene Synonyms/Alias
 GP110 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21GSRGASNKYLVEFRAacetylation[1]
21GSRGASNKYLVEFRAubiquitination[2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14]
34RAGKMSLKGTTVTPDubiquitination[2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16]
42GTTVTPDKRKGLVYIubiquitination[5, 6, 12]
62SLIHFCWKDRTSGNVubiquitination[5, 11, 14]
83FPDDCEFKRVPQCPSubiquitination[5, 6, 7, 11, 12]
97SGRVYVLKFKAGSKRubiquitination[4, 11, 12, 13, 14]
379GDVEAFAKAMQNNAKubiquitination[4, 5, 12, 13]
386KAMQNNAKPEQKEGDubiquitination[5, 12]
390NNAKPEQKEGDTKDKubiquitination[5]
395EQKEGDTKDKKDEEEubiquitination[5, 6, 12]
397KEGDTKDKKDEEEDMubiquitination[5, 6, 12, 14]
398EGDTKDKKDEEEDMSubiquitination[5, 12, 14]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [3] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [11] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [12] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [13] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [14] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [15] Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.
 Meierhofer D, Wang X, Huang L, Kaiser P.
 J Proteome Res. 2008 Oct;7(10):4566-76. [PMID: 18781797]
 [16] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
 Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity. 
Sequence Annotation
 DOMAIN 22 130 PH.
 REGION 2 132 Interaction with PSMD1.
 REGION 362 407 Interaction with UCHL5.
 MOD_RES 2 2 N-acetylthreonine.
 MOD_RES 211 211 Phosphoserine.
 MOD_RES 213 213 Phosphoserine (By similarity).
 MOD_RES 217 217 Phosphothreonine.
 CROSSLNK 34 34 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Isopeptide bond; Nucleus; Phosphoprotein; Proteasome; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 407 AA 
Protein Sequence
MTTSGALFPS LVPGSRGASN KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ QTDDSLIHFC 60
WKDRTSGNVE DDLIIFPDDC EFKRVPQCPS GRVYVLKFKA GSKRLFFWMQ EPKTDQDEEH 120
CRKVNEYLNN PPMPGALGAS GSSGHELSAL GGEGGLQSLL GNMSHSQLMQ LIGPAGLGGL 180
GGLGALTGPG LASLLGSSGP PGSSSSSSSR SQSAAVTPSS TTSSTRATPA PSAPAAASAT 240
SPSPAPSSGN GASTAASPTQ PIQLSDLQSI LATMNVPAGP AGGQQVDLAS VLTPEIMAPI 300
LANADVQERL LPYLPSGESL PQTADEIQNT LTSPQFQQAL GMFSAALASG QLGPLMCQFG 360
LPAEAVEAAN KGDVEAFAKA MQNNAKPEQK EGDTKDKKDE EEDMSLD 407 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000502; C:proteasome complex; IDA:UniProtKB.
 GO:0061133; F:endopeptidase activator activity; IDA:UniProtKB.
 GO:0070628; F:proteasome binding; IDA:UniProtKB.
 GO:0043248; P:proteasome assembly; IDA:UniProtKB.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:UniProtKB. 
Interpro
 IPR006773; 26S_Psome_Ubiquitin-recp_Rpn13. 
Pfam
 PF04683; Proteasom_Rpn13 
SMART
  
PROSITE
 PS50003; PH_DOMAIN 
PRINTS