UniProt Accession

 CDC27_HUMAN; P30260; G3V1C4; Q16349; Q96F35 

Genbank Protein ID

 U00001; S78234; AY518321; AC002558; CH471231; BC011656 

Genbank Nucleotide ID

 AAA60471.1; AAB34378.1; AAR89911.1; EAW57687.1; AAH11656.1 

Protein Name

 Cell division cycle protein 27 homolog 

Protein Synonyms/Alias

 Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC 

Gene Name

 CDC27 

Gene Synonyms/Alias

 ANAPC3; D0S1430E; D17S978E 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
99	LSGGVFNKQKSHDDI	ubiquitination	[1, 2]
330	RIGQTGTKSVFSQSG	ubiquitination	[2, 3]
415	KTKSKTNKGGITQPN	ubiquitination	[2]
431	NDSLEITKLDSSIIS	ubiquitination	[2, 4]
441	SSIISEGKISTITPQ	ubiquitination	[2]
557	VALSVLSKDLTDMDK	ubiquitination	[2]
588	REHDIAIKFFQRAIQ	ubiquitination	[1]
694	KALDTLNKAIVIDPK	ubiquitination	[1, 2]
718	SVLFANEKYKSALQE	ubiquitination	[2]
720	LFANEKYKSALQELE	ubiquitination	[2]
730	LQELEELKQIVPKES	ubiquitination	[2]
735	ELKQIVPKESLVYFL	ubiquitination	[1]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. 

Sequence Annotation

 REPEAT 84 114 TPR 1.
 REPEAT 115 148 TPR 2.
 REPEAT 499 532 TPR 3.
 REPEAT 567 600 TPR 4.
 REPEAT 602 634 TPR 5.
 REPEAT 635 668 TPR 6.
 REPEAT 670 702 TPR 7.
 REPEAT 704 736 TPR 8.
 REPEAT 737 770 TPR 9.
 MOD_RES 205 205 Phosphothreonine.
 MOD_RES 209 209 Phosphothreonine.
 MOD_RES 244 244 Phosphothreonine.
 MOD_RES 291 291 Phosphoserine.
 MOD_RES 313 313 Phosphothreonine.
 MOD_RES 339 339 Phosphoserine.
 MOD_RES 366 366 Phosphothreonine.
 MOD_RES 386 386 Phosphoserine.
 MOD_RES 426 426 Phosphoserine.
 MOD_RES 430 430 Phosphothreonine.
 MOD_RES 435 435 Phosphoserine.
 MOD_RES 438 438 Phosphoserine.
 MOD_RES 446 446 Phosphothreonine.
 MOD_RES 821 821 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 824 AA 

Protein Sequence

Gene Ontology

 GO:0005680; C:anaphase-promoting complex; IDA:UniProtKB.
 GO:0005813; C:centrosome; IDA:MGI.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005876; C:spindle microtubule; IDA:MGI.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; IMP:MGI.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
 GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0070979; P:protein K11-linked ubiquitination; IDA:UniProtKB. 

Interpro

 IPR026803; CDC27.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 

Pfam

 PF00515; TPR_1
 PF07719; TPR_2 

SMART

 SM00028; TPR 

PROSITE

 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS