UniProt Accession

 TRBP2_HUMAN; Q15633; Q12878; Q8WY32; Q8WY33; Q9BRY2 

Genbank Protein ID

 M60801; U08998; BT007140; CH471054; BC005860; AF281068; AF281068 

Genbank Nucleotide ID

 AAA36765.1; AAB50581.2; AAP35804.1; EAW96720.1; AAH05860.1; AAL55730.1; AAL55731.1 

Protein Name

 RISC-loading complex subunit TARBP2 

Protein Synonyms/Alias

 TAR RNA-binding protein 2; Trans-activation-responsive RNA-binding protein 

Gene Name

 TARBP2 

Gene Synonyms/Alias

 TRBP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
275	LRNSVGEKILSLRSC	ubiquitination	[1]
360	RRALQYLKIMAGSK*	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. 

Sequence Annotation

 DOMAIN 30 97 DRBM 1.
 DOMAIN 159 227 DRBM 2.
 DOMAIN 293 361 DRBM 3.
 REGION 22 105 Sufficient for interaction with PRKRA.
 REGION 152 234 Sufficient for interaction with PRKRA.
 REGION 228 366 Sufficient for interaction with DICER1.
 REGION 287 366 Sufficient for interaction with PRKRA.  

Keyword

 3D-structure; Alternative promoter usage; Complete proteome; Cytoplasm; Nucleus; Polymorphism; Reference proteome; Repeat; RNA-binding; RNA-mediated gene silencing; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 366 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016442; C:RNA-induced silencing complex; IDA:UniProtKB.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0035197; F:siRNA binding; IDA:UniProtKB.
 GO:0035280; P:miRNA loading onto RISC involved in gene silencing by miRNA; IDA:UniProtKB.
 GO:0050689; P:negative regulation of defense response to virus by host; IDA:UniProtKB.
 GO:0006469; P:negative regulation of protein kinase activity; TAS:UniProtKB.
 GO:0045070; P:positive regulation of viral genome replication; IDA:UniProtKB.
 GO:0031054; P:pre-miRNA processing; IDA:UniProtKB.
 GO:0030422; P:production of siRNA involved in RNA interference; IDA:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0046782; P:regulation of viral transcription; IDA:UniProtKB.
 GO:0030423; P:targeting of mRNA for destruction involved in RNA interference; IMP:UniProtKB. 

Interpro

 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom. 

Pfam

 PF00035; dsrm 

SMART

 SM00358; DSRM 

PROSITE

 PS50137; DS_RBD 

PRINTS