CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002555
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peroxisomal bifunctional enzyme 
Protein Synonyms/Alias
 PBE; PBFE; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase 
Gene Name
 Ehhadh 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
155LDLITSGKYLSADEAacetylation[1]
173GILDAVVKSDPVEEAacetylation[1]
182DPVEEAIKFAQKIIDacetylation[1]
186EAIKFAQKIIDKPIEacetylation[1]
190FAQKIIDKPIEPRRIacetylation[1]
218VFAEAIAKVRKQYPGacetylation[1]
241RSIQASVKHPYEVGIacetylation[1]
249HPYEVGIKEEEKLFMacetylation[1]
265LRASGQAKALQYAFFacetylation[1]
275QYAFFAEKSANKWSTacetylation[1]
279FAEKSANKWSTPSGAacetylation[1]
330VAVESDPKQLDAAKKacetylation[1]
337KQLDAAKKIITFTLEacetylation[1]
345IITFTLEKEASRAHQacetylation[1]
463ATVMSLSKKIGKIGVacetylation[1]
531AGLDVGWKIRKGQGLacetylation[1]
583KGWYQYDKPLGRIHKacetylation[1]
590KPLGRIHKPDPWLSTacetylation[1]
615IEQRTISKEEILERCacetylation[1]
680TVLEKLQKYYRQNPDacetylation[1]
709AQGSPPLKEWQSLAGacetylation[1]
721LAGPHGSKL******acetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 REGION 1 281 Enoyl-CoA hydratase / isomerase.
 REGION 282 571 3-hydroxyacyl-CoA dehydrogenase.
 MOTIF 720 722 Microbody targeting signal.
 BINDING 100 100 Substrate; via amide nitrogen (By
 MOD_RES 2 2 Blocked amino end (Ala).
 MOD_RES 241 241 N6-acetyllysine (By similarity).
 MOD_RES 330 330 N6-acetyllysine (By similarity).
 MOD_RES 345 345 N6-acetyllysine (By similarity).
 MOD_RES 583 583 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Isomerase; Lipid metabolism; Lyase; Multifunctional enzyme; NAD; Oxidoreductase; Peroxisome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
MAEYLRLPHS LAMIRLCNPP VNAVSPTVIR EVRNGLQKAG SDHTVKAIVI CGANGNFCAG 60
ADIHGFSAFT PGLALGSLVD EIQRYQKPVL AAIQGVALGG GLELALGCHY RIANAKARVG 120
LPEVTLGILP GARGTQLLPR VVGVPVALDL ITSGKYLSAD EALRLGILDA VVKSDPVEEA 180
IKFAQKIIDK PIEPRRIFNK PVPSLPNMDS VFAEAIAKVR KQYPGVLAPE TCVRSIQASV 240
KHPYEVGIKE EEKLFMYLRA SGQAKALQYA FFAEKSANKW STPSGASWKT ASAQPVSSVG 300
VLGLGTMGRG IAISFARVGI SVVAVESDPK QLDAAKKIIT FTLEKEASRA HQNGQASAKP 360
KLRFSSSTKE LSTVDLVVEA VFEDMNLKKK VFAELSALCK PGAFLCTNTS ALNVDDIASS 420
TDRPQLVIGT HFFSPAHVMR LLEVIPSRYS SPTTIATVMS LSKKIGKIGV VVGNCYGFVG 480
NRMLAPYYNQ GFFLLEEGSK PEDVDGVLEE FGFKMGPFRV SDLAGLDVGW KIRKGQGLTG 540
PSLPPGTPVR KRGNSRYSPL GDMLCEAGRF GQKTGKGWYQ YDKPLGRIHK PDPWLSTFLS 600
QYREVHHIEQ RTISKEEILE RCLYSLINEA FRILEEGMAA RPEHIDVIYL HGYGWPRHKG 660
GPMFYAASVG LPTVLEKLQK YYRQNPDIPQ LEPSDYLRRL VAQGSPPLKE WQSLAGPHGS 720
KL 722 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005777; C:peroxisome; IDA:UniProtKB.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IDA:RGD.
 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:EC.
 GO:0004300; F:enoyl-CoA hydratase activity; IEA:EC.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
 GO:0006475; P:internal protein amino acid acetylation; ISS:UniProtKB. 
Interpro
 IPR006180; 3-OHacyl-CoA_DH_CS.
 IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
 IPR006108; 3HC_DH_C.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR001753; Crotonase_core_superfam.
 IPR013328; DH_multihelical.
 IPR018376; Enoyl-CoA_hyd/isom_CS.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00725; 3HCDH
 PF02737; 3HCDH_N
 PF00378; ECH 
SMART
  
PROSITE
 PS00067; 3HCDH
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS