CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015627
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine-dependent NAD(+) synthetase 
Protein Synonyms/Alias
 NAD(+) synthase [glutamine-hydrolyzing]; NAD(+) synthetase; NH3-dependent NAD(+) synthetase-like protein 
Gene Name
 Nadsyn1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
28GNFQRILKSIQIAKGubiquitination[1]
544NPIGGISKTDLRAFVubiquitination[1]
609GRLRKVAKAGPYSMFubiquitination[1]
635TPTQVAEKVKLFFSKubiquitination[1]
637TQVAEKVKLFFSKYSubiquitination[1]
642KVKLFFSKYSMNRHKubiquitination[1]
649KYSMNRHKMTTLTPAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
 DOMAIN 5 294 CN hydrolase.
 NP_BIND 355 362 ATP (By similarity).
 REGION 325 706 Ligase (By similarity).
 ACT_SITE 357 357 By similarity.  
Keyword
 ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 725 AA 
Protein Sequence
MGRKVTVATC ALNQWALDFE GNFQRILKSI QIAKGKGARY RLGPELEICG YGCWDHYHES 60
DTLLHSLQVL AALLDSPVTQ DIICDVGMPI MHRNVRYNCR VIFLNRKILL IRPKMALANE 120
GNYRELRWFT PWTRSRQTEE YVLPRMLQDL TKQKTVPFGD VVLATQDTCV GSEICEELWT 180
PRSPHIDMGL DGVEIITNAS GSHHVLRKAH TRVDLVTMAT SKNGGIYLLA NQKGCDGDRL 240
YYDGCAMIAM NGSIFAQGTQ FSLDDVEVLT ATLDLEDVRS YKAEISSRNL EATRVSPYPR 300
VTVDFALSVS EDLLEPVSEP MEWTYHRPEE EISLGPACWL WDFLRRSKQA GFFLPLSGGV 360
DSAASACIVY SMCCLVCDAV KSGNQQVLTD VQNLVDESSY TPQDPRELCG RLLTTCYMAS 420
ENSSQETHSR ATKLAQLIGS YHINLSIDTA VKAVLGIFSL MTGKLPRFSA HGGSSRENLA 480
LQNVQARIRM VLAYLFAQLS LWSRGARGSL LVLGSANVDE SLLGYLTKYD CSSADINPIG 540
GISKTDLRAF VQFCAERFQL PVLQTILSAP ATAELEPLAD GQVSQMDEED MGMTYAELSI 600
FGRLRKVAKA GPYSMFCKLL NMWRDSYTPT QVAEKVKLFF SKYSMNRHKM TTLTPAYHAE 660
NYSPDDNRFD LRPFLYNTRW PWQFLCIDNQ VLQLERKASQ TREEQVLEHF KEPSPIWKQL 720
LPKDP 725 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IEA:InterPro.
 GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; ISA:MGI.
 GO:0008795; F:NAD+ synthase activity; ISA:MGI.
 GO:0009435; P:NAD biosynthetic process; ISA:MGI. 
Interpro
 IPR003010; C-N_Hydrolase.
 IPR014445; Gln-dep_NAD_synthase.
 IPR022310; NAD/GMP_synthase.
 IPR003694; NAD_synthase.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00795; CN_hydrolase
 PF02540; NAD_synthase 
SMART
  
PROSITE
 PS50263; CN_HYDROLASE 
PRINTS