UniProt Accession

 DDX21_MOUSE; Q9JIK5; Q3TVJ3; Q9WV45 

Genbank Protein ID

 AF220365; AF159131; AK160095; CH466553; BC043655 

Genbank Nucleotide ID

 AAF61690.1; AAD43959.3; BAE35625.1; EDL32086.1; AAH43655.1 

Protein Name

 Nucleolar RNA helicase 2 

Protein Synonyms/Alias

 DEAD box protein 21; Gu-alpha; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu 

Gene Name

 Ddx21 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
10	GKLRSGAKLGSDGAE	acetylation	[1, 2]
39	RKEKTKSKTEEATEG	acetylation	[1, 2]
217	ASGDAGEKSPRLKDG	acetylation	[2]
222	GEKSPRLKDGLSQPS	ubiquitination	[3]
358	DFSDITKKLSVACFY	acetylation	[4]
399	KDHLQNGKLDLTKLK	ubiquitination	[3]
459	HWVFNVAKKYMKSTY	ubiquitination	[3]
474	EQVDLIGKKTQKAAI	ubiquitination	[3]
521	IIFCETKKDAQELSQ	ubiquitination	[3]
546	LHGDIPQKQREITLK	ubiquitination	[3]
627	QLAQVEQKAGIKFKR	ubiquitination	[3]
645	PSATEIIKASSKDAI	ubiquitination	[3]
730	SYAWKELKEQLGESI	acetylation	[1]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription (By similarity). 

Sequence Annotation

 REPEAT 117 153 1-1.
 REPEAT 154 190 1-2.
 REPEAT 191 227 1-3.
 DOMAIN 289 468 Helicase ATP-binding.
 DOMAIN 501 645 Helicase C-terminal.
 REPEAT 807 811 2-1.
 REPEAT 817 823 2-2.
 REPEAT 829 833 2-3.
 NP_BIND 302 309 ATP (By similarity).
 REGION 117 227 3 X 37 AA tandem repeats.
 REGION 807 833 3 X 5 AA repeats.
 MOTIF 258 286 Q motif.
 MOTIF 411 414 DEVD box.
 MOD_RES 13 13 Phosphoserine (By similarity).
 MOD_RES 118 118 Phosphoserine.
 MOD_RES 144 144 Phosphoserine.
 MOD_RES 155 155 Phosphoserine.
 MOD_RES 192 192 Phosphoserine.
 MOD_RES 236 236 Phosphoserine.
 MOD_RES 244 244 Phosphoserine.
 MOD_RES 245 245 Phosphoserine.
 MOD_RES 247 247 Phosphothreonine.
 MOD_RES 261 261 Phosphoserine.
 MOD_RES 368 368 Phosphothreonine (By similarity).
 MOD_RES 639 639 Phosphoserine (By similarity).
 MOD_RES 847 847 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 851 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; ISS:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003725; F:double-stranded RNA binding; IDA:MGI.
 GO:0003724; F:RNA helicase activity; ISS:MGI.
 GO:0043330; P:response to exogenous dsRNA; IMP:MGI.
 GO:0009615; P:response to virus; IMP:MGI. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR012562; GUCT.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF08152; GUCT
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS