UniProt Accession

 I3L112_HUMAN; I3L112 

Genbank Protein ID

 AC015912; AC106858 

Genbank Nucleotide ID

  

Protein Name

 Diacylglycerol kinase epsilon 

Protein Synonyms/Alias

  

Gene Name

 DGKE 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
53	ADKRFQCKEIMLKND	ubiquitination	[1]
159	VLASKLGKQWTPLII	ubiquitination	[1, 2]
198	VQVFDVTKTPPIKAL	ubiquitination	[1, 2]
301	WKVQVTNKGYYNLRK	ubiquitination	[1, 2, 3]
336	NFHAHREKAPSLFSS	ubiquitination	[1]
348	FSSRILNKAVYLFYG	ubiquitination	[1]
357	VYLFYGTKDCLVQEC	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 456 AA 

Protein Sequence

Gene Ontology

 GO:0004143; F:diacylglycerol kinase activity; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro. 

Interpro

 IPR000756; Diacylglycerol_kin_accessory.
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd. 

Pfam

 PF00130; C1_1
 PF00609; DAGK_acc
 PF00781; DAGK_cat 

SMART

 SM00109; C1
 SM00045; DAGKa
 SM00046; DAGKc 

PROSITE

 PS50146; DAGK
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 

PRINTS