UniProt Accession

 KAD2_MOUSE; Q9WTP6; A2A820; Q3THT3; Q3TI11; Q3TKI6; Q8C7I9; Q9CY37 

Genbank Protein ID

 AB020202; AK010951; AK050133; AK166976; AK168056; AK168148; AL607086; AL607086; CU210866; CU210866; BC008610 

Genbank Nucleotide ID

 BAA77359.1; BAB27286.1; BAC34085.1; BAE39159.1; BAE40035.1; BAE40113.1; CAM19113.1; CAM19114.1; CAQ51906.1; CAQ51907.1; AAH08610.1 

Protein Name

 Adenylate kinase 2, mitochondrial 

Protein Synonyms/Alias

 AK 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Adenylate kinase 2, mitochondrial, N-terminally processed 

Gene Name

 Ak2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
14	ASEPEIPKGIRAVLL	acetylation	[1]
28	LGPPGAGKGTQAPKL	ubiquitination	[2]
34	GKGTQAPKLAENFCV	acetylation	[3]
85	MVVELIEKNLETPSC	acetylation	[4]
93	NLETPSCKNGFLLDG	acetylation	[5, 6, 7]
93	NLETPSCKNGFLLDG	succinylation	[6]
93	NLETPSCKNGFLLDG	ubiquitination	[2]
117	MLDDLMEKRKEKLDS	acetylation	[3, 7]
117	MLDDLMEKRKEKLDS	ubiquitination	[2]
164	NPPKEPMKDDITGEP	acetylation	[3, 7]
181	RRSDDNEKALKTRLE	acetylation	[3, 4, 7, 8]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 

Functional Description

 Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis (By similarity). 

Sequence Annotation

 NP_BIND 25 30 ATP (By similarity).
 NP_BIND 72 74 AMP (By similarity).
 NP_BIND 100 103 AMP (By similarity).
 NP_BIND 151 152 ATP (By similarity).
 REGION 45 74 NMPbind (By similarity).
 REGION 141 178 LID (By similarity).
 BINDING 46 46 AMP (By similarity).
 BINDING 51 51 AMP (By similarity).
 BINDING 107 107 AMP (By similarity).
 BINDING 142 142 ATP (By similarity).
 BINDING 175 175 AMP (By similarity).
 BINDING 186 186 AMP (By similarity).
 BINDING 214 214 ATP; via carbonyl oxygen (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 93 93 N6-acetyllysine.
 DISULFID 42 92 By similarity.  

Keyword

 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Disulfide bond; Kinase; Mitochondrion; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 239 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
 GO:0097226; C:sperm mitochondrial sheath; IDA:MGI.
 GO:0004017; F:adenylate kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006172; P:ADP biosynthetic process; IEA:Compara.
 GO:0046033; P:AMP metabolic process; IEA:Compara.
 GO:0046034; P:ATP metabolic process; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0046060; P:dATP metabolic process; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0006119; P:oxidative phosphorylation; IEA:Compara.
 GO:0097066; P:response to thyroid hormone stimulus; IEA:Compara. 

Interpro

 IPR006259; Adenyl_kin_sub.
 IPR000850; Adenylate_kin.
 IPR007862; Adenylate_kinase_lid-dom.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00406; ADK
 PF05191; ADK_lid 

SMART

  

PROSITE

 PS00113; ADENYLATE_KINASE 

PRINTS

 PR00094; ADENYLTKNASE.