CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006168
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 26S proteasome regulatory subunit RPN2 
Protein Synonyms/Alias
  
Gene Name
 RPN2 
Gene Synonyms/Alias
 SEN3; YIL075C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
132TKDIIDPKLTSIFERacetylation[1]
146RMIEKCLKASELKLAubiquitination[2]
173IESALKSKLDQDSTSubiquitination[2]
376ASLGVIHKGNLLEGKmethylation[3]
611RIVQLLSKSHNAHVRubiquitination[4]
689FLSVITNKHQEGLAKacetylation[1]
794MYEEASGKEVEKVATubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. 
Sequence Annotation
 REPEAT 366 399 PC 1.
 REPEAT 403 440 PC 2.
 REPEAT 445 479 PC 3.
 REPEAT 480 514 PC 4.
 REPEAT 516 549 PC 5.
 REPEAT 550 585 PC 6.
 REPEAT 586 618 PC 7.
 REPEAT 620 654 PC 8.
 REPEAT 655 692 PC 9.
 REPEAT 698 734 PC 10.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 801 801 Phosphothreonine.
 MOD_RES 932 932 Phosphothreonine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Phosphoprotein; Proteasome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 945 AA 
Protein Sequence
MSLTTAAPLL ALLRENQDSV KTYALESINN VVDQLWSEIS NELPDIEALY DDDTFSDREM 60
AALIASKVYY NLGEYESAVK YALAAKDRFD IDEKSQFVET IVSKSIEMYV QEASKQYTKD 120
EQFYTKDIID PKLTSIFERM IEKCLKASEL KLALGIALEG YRLDIIESAL KSKLDQDSTS 180
ENVKIINYLL TLAITTVTNS KFRSSILRKS FDFLMNMPNC DYLTLNKVVV NLNDAGLALQ 240
LFKKLKEEND EGLSAQIAFD LVSSASQQLL EILVTELTAQ GYDPALLNIL SGLPTCDYYN 300
TFLLNNKNID IGLLNKSKSS LDGKFSLFHT AVSVANGFMH AGTTDNSFIK ANLPWLGKAQ 360
NWAKFTATAS LGVIHKGNLL EGKKVMAPYL PGSRASSRFI KGGSLYGLGL IYAGFGRDTT 420
DYLKNIIVEN SGTSGDEDVD VLLHGASLGI GLAAMGSANI EVYEALKEVL YNDSATSGEA 480
AALGMGLCML GTGKPEAIHD MFTYSQETQH GNITRGLAVG LALINYGRQE LADDLITKML 540
ASDESLLRYG GAFTIALAYA GTGNNSAVKR LLHVAVSDSN DDVRRAAVIA LGFVLLRDYT 600
TVPRIVQLLS KSHNAHVRCG TAFALGIACA GKGLQSAIDV LDPLTKDPVD FVRQAAMIAL 660
SMILIQQTEK LNPQVADINK NFLSVITNKH QEGLAKFGAC VAQGIMNAGG RNVTIQLENA 720
DTGTLDTKSV VGLVMFSQFW YWFPLAHFLS LSFTPTTVIG IRGSDQAIPK FQMNCYAKED 780
AFSYPRMYEE ASGKEVEKVA TAVLSTTARA KARAKKTKKE KGPNEEEKKK EHEEKEKERE 840
TNKKGIKETK ENDEEFYKNK YSSKPYKVDN MTRILPQQSR YISFIKDDRF VPVRKFKGNN 900
GVVVLRDREP KEPVALIETV RQMKDVNAPL PTPFKVDDNV DFPSA 945 
Gene Ontology
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0030234; F:enzyme regulator activity; IEA:InterPro.
 GO:0043248; P:proteasome assembly; IGI:SGD.
 GO:0050790; P:regulation of catalytic activity; IEA:GOC.
 GO:0042176; P:regulation of protein catabolic process; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:SGD. 
Interpro
 IPR016642; 26S_Psome_Rpn2.
 IPR002015; APC_proteasome.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold. 
Pfam
 PF01851; PC_rep 
SMART
  
PROSITE
  
PRINTS