CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022846
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Spastin 
Protein Synonyms/Alias
 Spastic paraplegia 4 protein 
Gene Name
 SPAST 
Gene Synonyms/Alias
 ADPSP; FSP2; KIAA1083; SPG4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
206QPVLPFSKSQTDVYNubiquitination[1]
421KYVGEGEKLVRALFAubiquitination[1]
519ETRLLLLKNLLCKQGubiquitination[1]
532QGSPLTQKELAQLARubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. May also play a role in axon growth and the formation of axonal branches. 
Sequence Annotation
 DOMAIN 120 195 MIT.
 NP_BIND 382 389 ATP (Probable).
 REGION 1 300 Required for interaction with RTN1.
 REGION 1 194 Required for midbody localization.
 REGION 1 80 Required for interaction with ATL1.
 REGION 1 50 Required for nuclear localization.
 REGION 50 87 Required for interaction with SSNA1 and
 REGION 112 196 Sufficient for interaction with CHMP1B.
 REGION 114 200 Required for interaction with
 REGION 226 328 Sufficient for interaction with
 REGION 228 616 Sufficient for microtubule severing.
 REGION 270 328 Required for interaction with
 MOTIF 4 11 Nuclear localization signal.
 MOTIF 59 67 Nuclear export signal.
 MOTIF 309 312 Nuclear localization signal.
 MOD_RES 245 245 Phosphoserine.
 MOD_RES 268 268 Phosphoserine.
 MOD_RES 306 306 Phosphothreonine.  
Keyword
 3D-structure; Alternative initiation; Alternative promoter usage; Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Endoplasmic reticulum; Endosome; Hereditary spastic paraplegia; Hydrolase; Membrane; Microtubule; Neurodegeneration; Neurogenesis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 616 AA 
Protein Sequence
MNSPGGRGKK KGSGGASNPV PPRPPPPCLA PAPPAAGPAP PPESPHKRNL YYFSYPLFVG 60
FALLRLVAFH LGLLFVWLCQ RFSRALMAAK RSSGAAPAPA SASAPAPVPG GEAERVRVFH 120
KQAFEYISIA LRIDEDEKAG QKEQAVEWYK KGIEELEKGI AVIVTGQGEQ CERARRLQAK 180
MMTNLVMAKD RLQLLEKMQP VLPFSKSQTD VYNDSTNLAC RNGHLQSESG AVPKRKDPLT 240
HTSNSLPRSK TVMKTGSAGL SGHHRAPSYS GLSMVSGVKQ GSGPAPTTHK GTPKTNRTNK 300
PSTPTTATRK KKDLKNFRNV DSNLANLIMN EIVDNGTAVK FDDIAGQDLA KQALQEIVIL 360
PSLRPELFTG LRAPARGLLL FGPPGNGKTM LAKAVAAESN ATFFNISAAS LTSKYVGEGE 420
KLVRALFAVA RELQPSIIFI DEVDSLLCER REGEHDASRR LKTEFLIEFD GVQSAGDDRV 480
LVMGATNRPQ ELDEAVLRRF IKRVYVSLPN EETRLLLLKN LLCKQGSPLT QKELAQLARM 540
TDGYSGSDLT ALAKDAALGP IRELKPEQVK NMSASEMRNI RLSDFTESLK KIKRSVSPQT 600
LEAYIRWNKD FGDTTV 616 
Gene Ontology
 GO:0005813; C:centrosome; IEA:HAMAP.
 GO:0031410; C:cytoplasmic vesicle; IDA:MGI.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:HAMAP.
 GO:0030496; C:midbody; IEA:HAMAP.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0008017; F:microtubule binding; IDA:UniProtKB.
 GO:0008568; F:microtubule-severing ATPase activity; IDA:UniProtKB.
 GO:0007409; P:axonogenesis; IEA:HAMAP.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0007109; P:cytokinesis, completion of separation; IMP:UniProtKB.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:UniProtKB.
 GO:0001578; P:microtubule bundle formation; IDA:UniProtKB.
 GO:0051013; P:microtubule severing; IDA:UniProtKB.
 GO:0031117; P:positive regulation of microtubule depolymerization; IEA:HAMAP.
 GO:0034214; P:protein hexamerization; IDA:UniProtKB.
 GO:0051260; P:protein homooligomerization; IDA:UniProtKB. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR007330; MIT.
 IPR027417; P-loop_NTPase.
 IPR017179; Spastin. 
Pfam
 PF00004; AAA
 PF04212; MIT 
SMART
 SM00382; AAA
 SM00745; MIT 
PROSITE
 PS00674; AAA 
PRINTS