CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001486
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UBX domain-containing protein 7 
Protein Synonyms/Alias
  
Gene Name
 UBXN7 
Gene Synonyms/Alias
 KIAA0794; UBXD7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14SAASSALKGLIQQFTubiquitination[1, 2, 3, 4]
84VRAPIPQKQEILVEPubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
99EPLFGAPKRRRPARSubiquitination[1, 2, 3, 4, 6, 7, 8, 9]
133RNGGAIDKKLTTLADubiquitination[6, 10]
134NGGAIDKKLTTLADLubiquitination[1, 2, 3, 4, 6, 8, 10]
151PPIDLMHKGSFETAKubiquitination[1, 3, 4, 10]
158KGSFETAKECGQMQNubiquitination[3]
194VWSNEAVKNIIREHFubiquitination[1, 2, 3, 4]
221QRYIQFYKLGDFPYVubiquitination[6, 8, 11]
440AKLLALVKHVQSKGYubiquitination[1, 2, 3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.
 Meierhofer D, Wang X, Huang L, Kaiser P.
 J Proteome Res. 2008 Oct;7(10):4566-76. [PMID: 18781797]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572
Functional Description
  
Sequence Annotation
 DOMAIN 408 485 UBX.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 278 278 Phosphoserine.
 MOD_RES 280 280 Phosphoserine (By similarity).
 MOD_RES 285 285 Phosphoserine.
 MOD_RES 288 288 Phosphoserine.
 CROSSLNK 99 99 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Complete proteome; Isopeptide bond; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 489 AA 
Protein Sequence
MAAHGGSAAS SALKGLIQQF TTITGASESV GKHMLEACNN NLEMAVTMFL DGGGIAEEPS 60
TSSASVSTVR PHTEEEVRAP IPQKQEILVE PEPLFGAPKR RRPARSIFDG FRDFQTETIR 120
QEQELRNGGA IDKKLTTLAD LFRPPIDLMH KGSFETAKEC GQMQNKWLMI NIQNVQDFAC 180
QCLNRDVWSN EAVKNIIREH FIFWQVYHDS EEGQRYIQFY KLGDFPYVSI LDPRTGQKLV 240
EWHQLDVSSF LDQVTGFLGE HGQLDGLSSS PPKKCARSES LIDASEDSQL EAAIRASLQE 300
THFDSTQTKQ DSRSDEESES ELFSGSEEFI SVCGSDEEEE VENLAKSRKS PHKDLGHRKE 360
ENRRPLTEPP VRTDPGTATN HQGLPAVDSE ILEMPPEKAD GVVEGIDVNG PKAQLMLRYP 420
DGKREQITLP EQAKLLALVK HVQSKGYPNE RFELLTNFPR RKLSHLDYDI TLQEAGLCPQ 480
ETVFVQERN 489 
Gene Ontology
 GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:BHF-UCL.
 GO:0043130; F:ubiquitin binding; IDA:BHF-UCL.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:BHF-UCL. 
Interpro
 IPR012336; Thioredoxin-like_fold.
 IPR006577; UAS.
 IPR009060; UBA-like.
 IPR001012; UBX.
 IPR017346; UCP037991_UAS/UBX. 
Pfam
 PF00789; UBX 
SMART
 SM00594; UAS
 SM00166; UBX 
PROSITE
 PS50033; UBX 
PRINTS