CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031428
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Malic enzyme 
Protein Synonyms/Alias
  
Gene Name
 ME1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9DLQDRNEKLFYRVLTacetylation[1, 2, 3]
9DLQDRNEKLFYRVLTubiquitination[4, 5]
262KIWLVDSKGLIVKGRubiquitination[5, 6]
276RASLTQEKEKFAHEHubiquitination[6]
337ALSNPTSKAECSAEQubiquitination[7]
347CSAEQCYKITKGRAIubiquitination[7]
437TIRDVSLKIAEKIVKubiquitination[5, 6]
450VKDAYQEKTATVYPEubiquitination[5, 6, 7]
461VYPEPQNKEAFVRSQubiquitination[5, 6]
494EVQKIQTKVDQ****ubiquitination[6]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
 ACT_SITE 27 27 Proton donor (By similarity).
 ACT_SITE 98 98 Proton acceptor (By similarity).
 METAL 170 170 Divalent metal cation (By similarity).
 METAL 171 171 Divalent metal cation (By similarity).
 METAL 194 194 Divalent metal cation (By similarity).  
Keyword
 Complete proteome; Metal-binding; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 497 AA 
Protein Sequence
MDLQDRNEKL FYRVLTSDIE KFMPIVYTPT VGLACQQYSL VFRKPRGLFI TIHDRGHIAS 60
VLNAWPEDVI KAIVVTDGER ILGLGDLGCN GMGIPVGKLA LYTACGGMNP QECLPVILDV 120
GTENEELLKD PLYIGLRQRR VRGSEYDDFL DEFMEAVSSK YGMNCLIQFE DFANVNAFRL 180
LNKYRNQYCT FNDDIQGTAS VAVAGLLAAL RITKNKLSDQ TILFQGAGEA ALGIAHLIVM 240
ALEKEGLPKE KAIKKIWLVD SKGLIVKGRA SLTQEKEKFA HEHEEMKNLE AIVQEIKPTA 300
LIGVAAIGGA FSEQILKDMA AFNERPIIFA LSNPTSKAEC SAEQCYKITK GRAIFASGSP 360
FDPVTLPNGQ TLYPGQGNNS YVFPGVALGV VACGLRQITD NIFLTTAEVI AQQVSDKHLE 420
EGRLYPPLNT IRDVSLKIAE KIVKDAYQEK TATVYPEPQN KEAFVRSQMY STDYDQILPD 480
CYSWPEEVQK IQTKVDQ 497 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:InterPro.
 GO:0051262; P:protein tetramerization; IEA:Compara. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.