UniProt Accession

 KAR_YEAST; Q07551; D6VRM6 

Genbank Protein ID

 Z74172; BK006938 

Genbank Nucleotide ID

 CAA98692.1; DAA11736.1 

Protein Name

 NADPH-dependent alpha-keto amide reductase 

Protein Synonyms/Alias

 AKR-E; YKAR 

Gene Name

 YDL124W; D2240 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
89	NAIFLTDKYSPQIKM	acetylation	[1]
89	NAIFLTDKYSPQIKM	ubiquitination	[2]
235	YVKELSEKYIKSEAQ	acetylation	[1]
238	ELSEKYIKSEAQIIL	ubiquitination	[2]
300	PLRLYWNKLYGKYNY	acetylation	[1]
304	YWNKLYGKYNYAAQK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Reduces aromatic alpha-keto amides, aliphatic and aromatic alpha-keto esters, but not beta-keto esters. 

Sequence Annotation

 NP_BIND 208 268 NADP (By similarity).
 ACT_SITE 64 64 Proton donor (By similarity).
 BINDING 122 122 Substrate (By similarity).
 MOD_RES 123 123 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; NADP; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 312 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; IDA:SGD.
 GO:0051268; F:alpha-keto amide reductase activity; IDA:SGD.
 GO:0051269; F:alpha-keto ester reductase activity; IDA:SGD.
 GO:0043603; P:cellular amide metabolic process; IDA:SGD.
 GO:0006725; P:cellular aromatic compound metabolic process; IDA:SGD.
 GO:0042180; P:cellular ketone metabolic process; IDA:SGD.
 GO:0034599; P:cellular response to oxidative stress; IGI:SGD. 

Interpro

 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 

PRINTS

 PR00069; ALDKETRDTASE.