CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011809
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase GIN4 
Protein Synonyms/Alias
 Growth inhibitory protein 4 
Gene Name
 GIN4 
Gene Synonyms/Alias
 YDR507C; D9719.13 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
86EREIIIMKLLNHPNVubiquitination[1]
451KNFSSKKKLSTIVNQubiquitination[1]
534DDWEYIEKETKRTSSubiquitination[1]
772GNGVSQLKDSTATTAubiquitination[1]
886DVGDSTIKDKSALKLubiquitination[1]
992DMEPPLNKSVQKIREubiquitination[1]
1084NNLTLTGKLSSDNIFubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Serine/threonine-protein kinase which regulates the localization and the function of the septins during mitosis. Phosphorylates SHS1. 
Sequence Annotation
 DOMAIN 19 289 Protein kinase.
 NP_BIND 25 33 ATP (By similarity).
 ACT_SITE 156 156 Proton acceptor (By similarity).
 BINDING 48 48 ATP (By similarity).
 MOD_RES 406 406 Phosphoserine.
 MOD_RES 465 465 Phosphoserine.
 MOD_RES 471 471 Phosphoserine.
 MOD_RES 617 617 Phosphoserine.
 MOD_RES 689 689 Phosphoserine.
 MOD_RES 719 719 Phosphoserine.
 MOD_RES 883 883 Phosphoserine.
 MOD_RES 884 884 Phosphothreonine.
 MOD_RES 930 930 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1142 AA 
Protein Sequence
MAINGNSIPA IKDNTIGPWK LGETLGLGST GKVQLARNGS TGQEAAVKVI SKAVFNTGNV 60
SGTSIVGSTT PDALPYGIER EIIIMKLLNH PNVLRLYDVW ETNTDLYLVL EYAEKGELFN 120
LLVERGPLPE HEAIRFFRQI IIGVSYCHAL GIVHRDLKPE NLLLDHKYNI KIADFGMAAL 180
ETEGKLLETS CGSPHYAAPE IVSGIPYQGF ASDVWSCGVI LFALLTGRLP FDEEDGNIRT 240
LLLKVQKGEF EMPSDDEISR EAQDLIRKIL TVDPERRIKT RDILKHPLLQ KYPSIRDSKS 300
IRGLPREDTY LTPLSESNSS IDATILQNLV ILWHGRDPEG IKEKLREPGA NAEKTLYALL 360
YRFKCDTQKE LIKQQQVKKR QSISSVSVSP SKKVSTTPQR RRNRESLISV TSSRKKPISF 420
NKFTASSASS SNLTTPGSSK RLSKNFSSKK KLSTIVNQSS PTPASRNKRA SVINVEKNQK 480
RASIFSTTKK NKRSSRSIKR MSLIPSMKRE SVTTKLMSTY AKLAEDDDWE YIEKETKRTS 540
SNFATLIDEI FEYEKYEQIR KEKEELERKV REAKAREELE RRRRKQEEKE RARKLLEKED 600
LKRKQEELKK QIEIDISDLE QELSKHKEEK LDGNIRSISA PMENEEKNIN HLEVDIDNIL 660
RRRNFSLQTR PVSRLDPGIM FSSPTEEVSP VEPKRTENER LTTEKKILET IRRSKFLGSS 720
FNIDKELKLS KMEYPSIIAP QRLSEERVVS DSNDGYESLI LPKDGNGVSQ LKDSTATTAP 780
VSDGRLRKIS EIRVPQFTRK SRHFSESNKR LSVLSMYSTK ESFTNLVDIL KNGNLDVNNQ 840
QSQRIPTPRS ADDSEFLFET VNEEAEYTGN SSNDERLYDV GDSTIKDKSA LKLNFADRFN 900
GSNEAKQTDN LHLPILPPLN GDNELRKQNS QEGDQAHPKI KSMIPESGSS SHTEKEEENE 960
EKEEKKPEQH KQEEDQEKRE KVVDDMEPPL NKSVQKIREK NAGSQAKDHS KDHLKEHKQD 1020
KNTAIGNGSF FRKFSKSSDK TMELYAKISA KQLFNGLEKL LRGWTQYGLK NIKSHPNNLT 1080
LTGKLSSDNI FSLRSTLFEV NIYPRGKMSV VQFKKVSGSF KAVKKLVNEV ENVLNKEGVL 1140
QK 1142 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; IDA:SGD.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0007117; P:budding cell bud growth; IMP:SGD.
 GO:0046777; P:protein autophosphorylation; IDA:SGD.
 GO:0000135; P:septin checkpoint; IGI:SGD.
 GO:0000921; P:septin ring assembly; IGI:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS