UniProt Accession

 URA7_YEAST; P28274; D6VPW0 

Genbank Protein ID

 X53995; X78214; Z35800; BK006936 

Genbank Nucleotide ID

 CAA37941.1; CAA55055.1; CAA84859.1; DAA07080.1 

Protein Name

 CTP synthase 1 

Protein Synonyms/Alias

 CTP synthetase 1; UTP--ammonia ligase 1 

Gene Name

 URA7 

Gene Synonyms/Alias

 YBL039C; YBL0410 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
28	SSTGMLMKTLGLKVT	methylation	[1]
33	LMKTLGLKVTSIKID	ubiquitination	[2]
84	YLGVTLTKDHNITTG	ubiquitination	[2]
100	IYSHVIAKERKGDYL	acetylation	[3]
193	IHGEQKTKPTQAAIK	ubiquitination	[2]
289	RGLELLSKWKATTGN	acetylation	[3]
289	RGLELLSKWKATTGN	ubiquitination	[2]
311	VKIALVGKYTNLKDS	acetylation	[3]
316	VGKYTNLKDSYLSVI	acetylation	[3]
356	PEAQESNKTKFHEAW	acetylation	[3]
422	TRSVLGRKDSHSAEF	ubiquitination	[4, 5, 6]
448	VFMPEIDKETMGGSM	acetylation	[3]
576	DLEAGENKFNF****	ubiquitination	[2]

Reference

 [1] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [5] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [6] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. 

Sequence Annotation

 DOMAIN 305 559 Glutamine amidotransferase type-1.
 ACT_SITE 404 404 For GATase activity (By similarity).
 ACT_SITE 535 535 For GATase activity (By similarity).
 ACT_SITE 537 537 For GATase activity (By similarity).
 CROSSLNK 422 422 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 ATP-binding; Complete proteome; Direct protein sequencing; Glutamine amidotransferase; Isopeptide bond; Ligase; Nucleotide-binding; Pyrimidine biosynthesis; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 579 AA 

Protein Sequence

Gene Ontology

 GO:0097268; C:cytoophidium; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003883; F:CTP synthase activity; IDA:SGD.
 GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006241; P:CTP biosynthetic process; IDA:SGD.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0008654; P:phospholipid biosynthetic process; IMP:SGD.
 GO:0019856; P:pyrimidine nucleobase biosynthetic process; IMP:SGD. 

Interpro

 IPR004468; CTP_synthase.
 IPR017456; CTP_synthase_N.
 IPR017926; GATASE.
 IPR027417; P-loop_NTPase. 

Pfam

 PF06418; CTP_synth_N
 PF00117; GATase 

SMART

  

PROSITE

 PS51273; GATASE_TYPE_1 

PRINTS