CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003907
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Medium-chain specific acyl-CoA dehydrogenase, mitochondrial 
Protein Synonyms/Alias
 MCAD 
Gene Name
 ACADM 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
212ARSDPDPKAPANKAFacetylation[1]
279VAMGAFDKTRPVVAAacetylation[1]
301RALDEATKYALERKTacetylation[1]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 This enzyme is specific for acyl chain lengths of 4 to 16. 
Sequence Annotation
 NP_BIND 158 167 FAD.
 NP_BIND 191 193 FAD.
 NP_BIND 306 308 FAD.
 NP_BIND 316 317 FAD.
 NP_BIND 374 378 FAD.
 NP_BIND 403 405 FAD.
 REGION 278 281 Substrate binding.
 ACT_SITE 401 401 Proton acceptor.
 BINDING 167 167 Substrate; via carbonyl oxygen.
 BINDING 216 216 Substrate.
 BINDING 402 402 Substrate; via amide nitrogen.
 BINDING 413 413 Substrate.
 MOD_RES 279 279 N6-acetyllysine.
 MOD_RES 301 301 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; FAD; Fatty acid metabolism; Flavoprotein; Lipid metabolism; Mitochondrion; Oxidoreductase; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 421 AA 
Protein Sequence
MAAGFGRCCR VLRSISRFHW RSQHTKANRQ REPGLGFSFE FTEQQKEFQA TARKFAREEI 60
IPVAAEYDKT GEYPVPLIRR AWELGLMNTH IPENCGGLGL GTFDACLISE ELAYGCTGVQ 120
TAIEGNSLGQ MPIIIAGNDQ QKKKYLGRMT EEPLMCAYCV TEPGAGSDVA GIKTKAEKKG 180
DEYIINGQKM WITNGGKANW YFLLARSDPD PKAPANKAFT GFIVEADTPG IQIGRKELNM 240
GQRCSDTRGI VFEDVKVPKE NVLIGDGAGF KVAMGAFDKT RPVVAAGAVG LAQRALDEAT 300
KYALERKTFG KLLVEHQAIS FMLAEMAMKV ELARMSYQRA AWEVDSGRRN TYYASIAKAF 360
AGDIANQLAT DAVQILGGNG FNTEYPVEKL MRDAKIYQIY EGTSQIQRLI VAREHIDKYK 420
N 421 
Gene Ontology
 GO:0030424; C:axon; IDA:UniProtKB.
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0042802; F:identical protein binding; IDA:BHF-UCL.
 GO:0070991; F:medium-chain-acyl-CoA dehydrogenase activity; IDA:BHF-UCL.
 GO:0055007; P:cardiac muscle cell differentiation; IEA:Compara.
 GO:0045329; P:carnitine biosynthetic process; IMP:BHF-UCL.
 GO:0019254; P:carnitine metabolic process, CoA-linked; IMP:BHF-UCL.
 GO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IDA:BHF-UCL.
 GO:0005978; P:glycogen biosynthetic process; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0051793; P:medium-chain fatty acid catabolic process; IDA:BHF-UCL.
 GO:0009791; P:post-embryonic development; IEA:Compara.
 GO:0006111; P:regulation of gluconeogenesis; IEA:Compara.
 GO:0009409; P:response to cold; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara. 
Interpro
 IPR006089; Acyl-CoA_DH_CS.
 IPR006092; Acyl-CoA_DH_N.
 IPR006090; Acyl-CoA_Oxase/DH_1.
 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 
Pfam
 PF00441; Acyl-CoA_dh_1
 PF02770; Acyl-CoA_dh_M
 PF02771; Acyl-CoA_dh_N 
SMART
  
PROSITE
 PS00072; ACYL_COA_DH_1
 PS00073; ACYL_COA_DH_2 
PRINTS