UniProt Accession

 AMPN_MOUSE; P97449; Q91YH8; Q99K63 

Genbank Protein ID

 U77083; BC005431; BC017011; BC040792 

Genbank Nucleotide ID

 AAB19065.1; AAH05431.1; AAH17011.1; AAH40792.1 

Protein Name

 Aminopeptidase N 

Protein Synonyms/Alias

 AP-N; mAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Membrane protein p161; Microsomal aminopeptidase; CD13 

Gene Name

 Anpep 

Gene Synonyms/Alias

 Lap-1; Lap1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
496	FLTEDLFKKGLSSYL	acetylation	[1]

Reference

 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

 Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May have a role in angiogenesis (By similarity). Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells. 

Sequence Annotation

 REGION 33 68 Cytosolic Ser/Thr-rich junction.
 REGION 69 966 Metalloprotease.
 REGION 351 355 Substrate binding (By similarity).
 ACT_SITE 388 388 Proton acceptor (By similarity).
 METAL 387 387 Zinc; catalytic (By similarity).
 METAL 391 391 Zinc; catalytic (By similarity).
 METAL 410 410 Zinc; catalytic (By similarity).
 MOD_RES 176 176 Sulfotyrosine (Potential).
 MOD_RES 418 418 Sulfotyrosine (Potential).
 MOD_RES 423 423 Sulfotyrosine (Potential).
 MOD_RES 852 852 Phosphotyrosine.
 CARBOHYD 106 106 N-linked (GlcNAc...).
 CARBOHYD 114 114 N-linked (GlcNAc...).
 CARBOHYD 128 128 N-linked (GlcNAc...).
 CARBOHYD 234 234 N-linked (GlcNAc...) (Potential).
 CARBOHYD 288 288 N-linked (GlcNAc...) (Potential).
 CARBOHYD 318 318 N-linked (GlcNAc...) (Potential).
 CARBOHYD 332 332 N-linked (GlcNAc...).
 CARBOHYD 573 573 N-linked (GlcNAc...) (Potential).
 CARBOHYD 606 606 N-linked (GlcNAc...).
 CARBOHYD 624 624 N-linked (GlcNAc...) (Potential).
 CARBOHYD 666 666 N-linked (GlcNAc...); atypical.
 CARBOHYD 734 734 N-linked (GlcNAc...) (Potential).
 CARBOHYD 783 783 N-linked (GlcNAc...); atypical.
 CARBOHYD 784 784 N-linked (GlcNAc...).
 CARBOHYD 785 785 N-linked (GlcNAc...); atypical.
 CARBOHYD 790 790 N-linked (GlcNAc...); atypical.
 CARBOHYD 817 817 N-linked (GlcNAc...).
 DISULFID 760 767 By similarity.
 DISULFID 797 833 By similarity.  

Keyword

 Aminopeptidase; Angiogenesis; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Phosphoprotein; Protease; Reference proteome; Signal-anchor; Sulfation; Transmembrane; Transmembrane helix; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 966 AA 

Protein Sequence

Gene Ontology

 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:Compara.
 GO:0009897; C:external side of plasma membrane; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
 GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR024571; DUF3358.
 IPR001930; Peptidase_M1.
 IPR014782; Peptidase_M1_N. 

Pfam

 PF11838; DUF3358
 PF01433; Peptidase_M1 

SMART

  

PROSITE

 PS00142; ZINC_PROTEASE 

PRINTS

 PR00756; ALADIPTASE.