CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020316
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rab11 family-interacting protein 5 
Protein Synonyms/Alias
 Rab11-FIP5; Gamma-SNAP-associated factor 1; Gaf-1; Phosphoprotein pp75; Rab11-interacting protein Rip11 
Gene Name
 RAB11FIP5 
Gene Synonyms/Alias
 GAF1; KIAA0857; RIP11 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
558ALSSGLEKLKTVTSGubiquitination[1, 2]
560SSGLEKLKTVTSGSIubiquitination[1, 3]
583AGQMVDTKRLKDSAVubiquitination[1, 2, 3, 4, 5]
586MVDTKRLKDSAVLDQubiquitination[1, 2, 3, 4, 5, 6, 7]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. 
Sequence Annotation
 DOMAIN 6 128 C2.
 DOMAIN 586 648 FIP-RBD.
 MOD_RES 176 176 Phosphoserine.
 MOD_RES 306 306 Phosphotyrosine (By similarity).
 MOD_RES 307 307 Phosphoserine.
 MOD_RES 494 494 Phosphoserine.
 MOD_RES 538 538 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Endosome; Membrane; Mitochondrion; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 653 AA 
Protein Sequence
MALVRGAEPA AGPSRWLPTH VQVTVLRARG LRGKSSGAGS TSDAYTVIQV GREKYSTSVV 60
EKTHGCPEWR EECSFELPPG ALDGLLRAQE ADAGPAPWAA SSAAACELVL TTMHRSLIGV 120
DKFLGQATVA LDEVFGAGRA QHTQWYKLHS KPGKKEKERG EIEVTIQFTR NNLSASMFDL 180
SMKDKPRSPF SKIRDKMKGK KKYDLESASA ILPSSAIEDP DLGSLGKMGK AKGFFLRNKL 240
RKSSLTQSNT SLGSDSTLSS ASGSLAYQGP GAELLTRSPS RSSWLSTEGG RDSAQSPKLF 300
THKRTYSDEA NQMRVAPPRA LLDLQGHLDA ASRSSLCVNG SHIYNEEPQG PVRHRSSISG 360
SLPSSGSLQA VSSRFSEEGP RSTDDTWPRG SRSNSSSEAV LGQEELSAQA KVLAPGASHP 420
GEEEGARLPE GKPVQVATPI VASSEAVAEK EGARKEERKP RMGLFHHHHQ GLSRSELGRR 480
SSLGEKGGPI LGASPHHSSS GEEKAKSSWF GLREAKDPTQ KPSPHPVKPL SAAPVEGSPD 540
RKQSRSSLSI ALSSGLEKLK TVTSGSIQPV TQAPQAGQMV DTKRLKDSAV LDQSAKYYHL 600
THDELISLLL QRERELSQRD EHVQELESYI DRLLVRIMET SPTLLQIPPG PPK 653 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005741; C:mitochondrial outer membrane; IDA:UniProtKB.
 GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
 GO:0043015; F:gamma-tubulin binding; IDA:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR019018; Rab-bd_FIP-RBD. 
Pfam
 PF00168; C2
 PF09457; RBD-FIP 
SMART
 SM00239; C2 
PROSITE
 PS50004; C2
 PS51511; FIP_RBD 
PRINTS