CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023333
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription elongation factor SPT5 
Protein Synonyms/Alias
 DRB sensitivity-inducing factor large subunit; DSIF large subunit; dSpt5 
Gene Name
 Spt5 
Gene Synonyms/Alias
 CG7626 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
744RDREILGKTIKISGGacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II. DSIF enhances transcriptional pausing at sites proximal to the promoter, which may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF may also promote transcriptional elongation within coding regions. DSIF is required for the transcriptional induction of heat shock response genes and regulation of genes which control anterior-posterior patterning during embryonic development. 
Sequence Annotation
 DOMAIN 309 336 KOW 1.
 DOMAIN 460 487 KOW 2.
 DOMAIN 513 545 KOW 3.
 DOMAIN 635 668 KOW 4.
 DOMAIN 740 773 KOW 5.
 REGION 213 307 Interaction with Spt4 (By similarity).
 REGION 350 461 Interaction with RNA polymerase II (By
 MOD_RES 73 73 Phosphoserine.
 MOD_RES 76 76 Phosphoserine.
 MOD_RES 90 90 Phosphoserine.
 MOD_RES 162 162 Phosphothreonine.
 MOD_RES 707 707 Phosphoserine.
 MOD_RES 808 808 Phosphothreonine.
 MOD_RES 815 815 Phosphothreonine; by CDK9 (By
 MOD_RES 825 825 Phosphothreonine; by CDK9 (By
 MOD_RES 847 847 Phosphothreonine.
 MOD_RES 855 855 Phosphothreonine.
 MOD_RES 867 867 Phosphothreonine.
 MOD_RES 868 868 Phosphothreonine.  
Keyword
 Activator; Alternative splicing; Chromosome; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1078 AA 
Protein Sequence
MSDSEVSNMS DSGSEDGSIS NKSQRSARSK SRSRSRSGSR GSRSVSRSRS RSQSGHSRSG 60
SESPQRRDNR GKSDESGEEE EEPPGEDIDS EEYDEEENDD HPRKKKKKER FGGFIIDEAE 120
VDDEVDEDDE WEEGANEIGI VGNEIDELGP TARDIEIRRR GTNLWDTQKE DEIEEYLRKK 180
YADESIAKRH FGDGGEEMSD EITQQTLLPG IKDPNLWMVK CRIGEEKATA LLLMRKYLTY 240
LNTDDPLQIK SIIAPEGVKG YIYLEAYKQT HVKTCIDNVG NLRMGKWKQE MVPIKEMTDV 300
LKVVKEQVGL KVKQWVRLKR GLYKDDIAQV DYVDLAQNQV HLKLLPRIDY TRMRGALRTT 360
ATESDDSKRK KKRRPAAKPF DPEAVRAIGG EVHSDGDFLL FEGNRYSRKG FLYKNFTMSA 420
ILSDGVKPTL AELERFEESP EEVNLEIMGT VKDDPTMAHS FSMGDNVEVC VGDLENLQAK 480
IVAIDGTMIT VMPKHQDLKD PLIFKASELR KYFKTGDHAR VLAGRYEGET GLIIRVEPTR 540
VVLVSDLTNH ELEVLPRDLQ LCSDVATGVD CLGQFQWGDM VQLDSQNVGV IVRLERENFH 600
VLGMNGKCIE CKPTALHKRK ENRHTVALDA DQNQIRRRDV VKVMEGPHAG RSGEIKHLYR 660
SLAFLHCRMY TENGGIFVCK TRHLQLAGGS KTTVSNAGIV GGLGFMSPRI QSPMHPSGGR 720
GARGGARGGR GGFRVTRDRE ILGKTIKISG GPYKGAVGIV KDATESTARV ELHTSCQTIS 780
VDRNHIAIVG VTGKEGSVST YGRTPARTPG YGAQTPSYTA AGSKTPLVGS QTPNWDTDTR 840
TPYGTMTPSH DGSMTPRHGA WDPTANTTPA RNNDFDYSLE EPSPSPGYNP STPGYQMTSQ 900
FAPQTPGTLY GSDRSYSPFN PSPSPAPSPY PVGYMNTPSP STYSPNTPGG IPQSPYNPQT 960
PGASLDSSMG DWCTTDIEVR IHTHDDTDLV GQTGIIRTVS NGVCSVFLRQ EDRSVSIVSE 1020
HLAPVLPCNG DEFKIIYGDD RESVGRVLSK DGDVFVCRIN EEIKLLPINF LCKMKSID 1078 
Gene Ontology
 GO:0032044; C:DSIF complex; ISS:UniProtKB.
 GO:0005705; C:polytene chromosome interband; IDA:UniProtKB.
 GO:0005703; C:polytene chromosome puff; IDA:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
 GO:0007095; P:mitotic G2 DNA damage checkpoint; IGI:FlyBase.
 GO:0007052; P:mitotic spindle organization; IMP:FlyBase.
 GO:0032785; P:negative regulation of DNA-dependent transcription, elongation; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0032786; P:positive regulation of DNA-dependent transcription, elongation; ISS:UniProtKB.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:InterPro.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IDA:UniProtKB. 
Interpro
 IPR005824; KOW.
 IPR024945; Spt5_C_dom.
 IPR022581; Spt5_N.
 IPR017071; TF_Spt5.
 IPR005100; TF_Spt5_NGN-domain.
 IPR006645; Transcrpt_antiterm_NusG_N.
 IPR008991; Translation_prot_SH3-like. 
Pfam
 PF00467; KOW
 PF03439; Spt5-NGN
 PF11942; Spt5_N 
SMART
 SM01104; CTD
 SM00739; KOW
 SM00738; NGN 
PROSITE
  
PRINTS