UniProt Accession

 LDHA_MOUSE; P06151 

Genbank Protein ID

 Y00309; X02520; X02521; X02522; X02523; X02524; X02525; X02526; U13687; X03753; M17516 

Genbank Nucleotide ID

 CAA68410.1; CAA26360.1; CAA26360.1; CAA26360.1; CAA26360.1; CAA26360.1; CAA26360.1; CAA26360.1; AAA21466.1; CAA27387.1; AAA39424.1 

Protein Name

 L-lactate dehydrogenase A chain 

Protein Synonyms/Alias

 LDH-A; LDH muscle subunit; LDH-M 

Gene Name

 Ldha 

Gene Synonyms/Alias

 Ldh-1; Ldh1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
5	***MATLKDQLIVNL	acetylation	[1]
76	SLFLKTPKIVSSKDY	ubiquitination	[2]
81	TPKIVSSKDYCVTAN	acetylation	[1]
224	ELGTDADKEQWKEVH	acetylation	[1]
228	DADKEQWKEVHKQVV	acetylation	[1]
232	EQWKEVHKQVVDSAY	acetylation	[1]
243	DSAYEVIKLKGYTSW	ubiquitination	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
 Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
 Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144] 

Functional Description

  

Sequence Annotation

 NP_BIND 29 57 NAD (By similarity).
 ACT_SITE 193 193 Proton acceptor (By similarity).
 BINDING 99 99 NAD (By similarity).
 BINDING 106 106 Substrate (By similarity).
 BINDING 138 138 NAD or substrate (By similarity).
 BINDING 169 169 Substrate (By similarity).
 BINDING 248 248 Substrate (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 5 5 N6-acetyllysine (By similarity).
 MOD_RES 14 14 N6-acetyllysine (By similarity).
 MOD_RES 57 57 N6-acetyllysine (By similarity).
 MOD_RES 81 81 N6-acetyllysine (By similarity).
 MOD_RES 118 118 N6-acetyllysine (By similarity).
 MOD_RES 126 126 N6-acetyllysine (By similarity).
 MOD_RES 239 239 Phosphotyrosine.
 MOD_RES 318 318 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 332 AA 

Protein Sequence

Gene Ontology

 GO:0005929; C:cilium; IDA:MGI.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004459; F:L-lactate dehydrogenase activity; IDA:MGI.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0031668; P:cellular response to extracellular stimulus; IDA:MGI.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR011304; L-lactate_DH.
 IPR018177; L-lactate_DH_AS.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00064; L_LDH 

PRINTS

 PR00086; LLDHDRGNASE.