CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002469
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-isopropylmalate dehydratase 
Protein Synonyms/Alias
 Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase 
Gene Name
 LEU1 
Gene Synonyms/Alias
 YGL009C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
81TESRKNFKSLDTFIKacetylation[1]
88KSLDTFIKQTDSRLQacetylation[1]
97TDSRLQVKTLENNVKacetylation[1]
104KTLENNVKQFGVPYFacetylation[1]
348LEPNTPLKSIKVDKVacetylation[1]
482DIREFEYKDQDQSSPacetylation[1]
571DTDAIIPKQFLKTIKacetylation[1]
584IKRTGLKKGLFYEWRacetylation[1]
639EHAPWALKDFGIKSIacetylation[1]
677DQQIIIDKLIPIANKacetylation[1]
746YEALRREKYSFLEGGacetylation[1]
755SFLEGGSKLLKFDNVacetylation[1]
758EGGSKLLKFDNVPKRacetylation[1]
774AVTTTFDKVHQDW**acetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. 
Sequence Annotation
 METAL 360 360 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 421 421 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 424 424 Iron-sulfur (4Fe-4S) (By similarity).
 MOD_RES 488 488 Phosphoserine.
 MOD_RES 494 494 Phosphothreonine.
 MOD_RES 495 495 Phosphoserine.  
Keyword
 4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Iron; Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 779 AA 
Protein Sequence
MVYTPSKGPR TLYDKVFDAH VVHQDENGSF LLYIDRHLVH EVTSPQAFEG LENAGRKVRR 60
VDCTLATVDH NIPTESRKNF KSLDTFIKQT DSRLQVKTLE NNVKQFGVPY FGMSDARQGI 120
VHTIGPEEGF TLPGTTVVCG DSHTSTHGAF GSLAFGIGTS EVEHVLATQT IIQAKSKNMR 180
ITVNGKLSPG ITSKDLILYI IGLIGTAGGT GCVIEFAGEA IEALSMEARM SMCNMAIEAG 240
ARAGMIKPDE TTFQYTKGRP LAPKGAEWEK AVAYWKTLKT DEGAKFDHEI NIEAVDVIPT 300
ITWGTSPQDA LPITGSVPDP KNVTDPIKKS GMERALAYMG LEPNTPLKSI KVDKVFIGSC 360
TNGRIEDLRS AAAVVRGQKL ASNIKLAMVV PGSGLVKKQA EAEGLDKIFQ EAGFEWREAG 420
CSICLGMNPD ILDAYERCAS TSNRNFEGRQ GALSRTHLMS PAMAAAAGIA GHFVDIREFE 480
YKDQDQSSPK VEVTSEDEKE LESAAYDHAE PVQPEDAPQD IANDELKDIP VKSDDTPAKP 540
SSSGMKPFLT LEGISAPLDK ANVDTDAIIP KQFLKTIKRT GLKKGLFYEW RFRKDDQGKD 600
QETDFVLNVE PWREAEILVV TGDNFGCGSS REHAPWALKD FGIKSIIAPS YGDIFYNNSF 660
KNGLLPIRLD QQIIIDKLIP IANKGGKLCV DLPNQKILDS DGNVLVDHFE IEPFRKHCLV 720
NGLDDIGITL QKEEYISRYE ALRREKYSFL EGGSKLLKFD NVPKRKAVTT TFDKVHQDW 779 
Gene Ontology
 GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0003861; F:3-isopropylmalate dehydratase activity; IMP:SGD.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009098; P:leucine biosynthetic process; IMP:SGD. 
Interpro
 IPR004430; 3-IsopropMal_deHydase_lsu.
 IPR004431; 3-IsopropMal_deHydase_ssu.
 IPR012235; 3-IsopropMal_deHydtase_ssu/lsu.
 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015928; Aconitase/3IPM_dehydase_swvl.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS.
 IPR000573; AconitaseA/IPMdHydase_ssu_swvl. 
Pfam
 PF00330; Aconitase
 PF00694; Aconitase_C 
SMART
  
PROSITE
 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 
PRINTS
 PR00415; ACONITASE.