CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010382
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 RNA polymerase sigma factor SigA 
Protein Synonyms/Alias
 Sigma-A 
Gene Name
 sigA 
Gene Synonyms/Alias
 rpoD; MPN_352; MP484 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
71LKTLQENKESDVPKKacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth (By similarity). 
Sequence Annotation
 DNA_BIND 453 472 H-T-H motif (By similarity).
 REGION 252 322 Sigma-70 factor domain-2 (By similarity).
 REGION 331 412 Sigma-70 factor domain-3 (By similarity).
 REGION 425 480 Sigma-70 factor domain-4 (By similarity).
 MOTIF 276 279 Interaction with polymerase core subunit  
Keyword
 Complete proteome; Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 499 AA 
Protein Sequence
MSSPKKNFKK PQPKTENQKQ ALNEERIAEL KKSRILGKNR PFKKMIYVDT KAQRKQKHEN 60
VAFLKTLQEN KESDVPKKRR GRKPKHAPLK EKNNLKLFDI LEGSLKSHTE NDDTNKVISL 120
LVEVWEKKNK KKDNNSLSNK DIVNVLSKFE LPDDEIIFVL DELRDKGIEL PHDVEEHIHE 180
FRANQDLSII DEDIEELTTK NISNRDKVDD NVRFFLGSLD SSKMLDFESE QRIAKVLNST 240
DEESRKYAIN QLVTSNLRLV VSIAKKHLER GLDFNDLIQE GNLGLLKAIS KFNWSLGNKF 300
STYATWWIKQ AITRAIADQA RTVRIPVHMV ETINRLAKAE RALNQELGRE PTAEELAEKM 360
GGQAEGFTVK KIAEIKRLSL DPVSLDKTVG HDEESQFGDF VRDTDAQMPD EFTESRSNYE 420
KIDELLNNCL SEQEELIVRM RIGMPPYNET KTLDEVSQKI KIPREKIRQI ETKAIRKLRQ 480
AVRNNHMSLS FMRGNEKKD 499 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
 GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro. 
Interpro
 IPR014284; RNA_pol_sigma-70_dom.
 IPR000943; RNA_pol_sigma70.
 IPR007627; RNA_pol_sigma70_r2.
 IPR007624; RNA_pol_sigma70_r3.
 IPR007630; RNA_pol_sigma70_r4.
 IPR013325; RNA_pol_sigma_r2.
 IPR013324; RNA_pol_sigma_r3_r4.
 IPR012760; RNA_pol_sigma_RpoD_C.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF04542; Sigma70_r2
 PF04539; Sigma70_r3
 PF04545; Sigma70_r4 
SMART
  
PROSITE
 PS00715; SIGMA70_1
 PS00716; SIGMA70_2 
PRINTS
 PR00046; SIGMA70FCT.