CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015832
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase HUWE1 
Protein Synonyms/Alias
 E3Histone; HECT, UBA and WWE domain-containing protein 1; Upstream regulatory element-binding protein 1; URE-B1; URE-binding protein 1 
Gene Name
 Huwe1 
Gene Synonyms/Alias
 Kiaa0312; Ureb1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
25DCRALIDKLKVCNDEubiquitination[1]
324DKQLMEIKAASLRTLubiquitination[2]
342VHLERTPKLSSIIDCubiquitination[1]
632QPFERLFKVLLSPDYubiquitination[1]
674MRHQPTLKTDATTAIubiquitination[1]
1107STASALTKLLTKGLSubiquitination[1, 2]
1111ALTKLLTKGLSWQPPubiquitination[1, 2]
1403PEEVACRKEEEERKAubiquitination[1]
1470DLIMTAIKRNGADYRubiquitination[1, 2]
1608KRRAQMTKYLQSNSNubiquitination[1, 2]
1643STIDSAWKSGETSVRubiquitination[1]
1840TLRHTMEKVVRSAATubiquitination[1]
2172AMAESTEKHARLQAVubiquitination[1]
2219IIRLFLKKGLVNDLAubiquitination[1]
2248NTVNAALKPLETLSRubiquitination[1]
3622GTRDTVLKLLLNGARubiquitination[1]
3637HLGYTLCKQIGTLLAubiquitination[1]
3676EEQPQTTKLKGKMQSubiquitination[1]
3678QPQTTKLKGKMQSRFubiquitination[1]
3698VVIVASQKRPLGGREubiquitination[1]
3717SMSMLTSKTSTQKFFubiquitination[1]
3722TSKTSTQKFFLRVLQubiquitination[1]
3915ESQLAHIKDEPPPLSubiquitination[1]
4000RVLDFDVKRKYFRQEubiquitination[1]
4002LDFDVKRKYFRQELEubiquitination[1]
4017RLDEGLRKEDMAVHVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene (By similarity). Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation (By similarity). 
Sequence Annotation
 DOMAIN 1316 1355 UBA.
 REPEAT 1370 1389 UIM.
 DOMAIN 1603 1680 WWE.
 DOMAIN 4041 4377 HECT.
 ACT_SITE 4344 4344 Glycyl thioester intermediate (By
 MOD_RES 1084 1084 Phosphoserine (By similarity).
 MOD_RES 1368 1368 Phosphoserine (By similarity).
 MOD_RES 1370 1370 Phosphoserine (By similarity).
 MOD_RES 1395 1395 Phosphoserine (By similarity).
 MOD_RES 1907 1907 Phosphoserine.
 MOD_RES 2362 2362 Phosphoserine (By similarity).
 MOD_RES 2365 2365 Phosphoserine (By similarity).
 MOD_RES 2391 2391 Phosphoserine (By similarity).
 MOD_RES 2595 2595 Phosphoserine (By similarity).
 MOD_RES 2619 2619 Phosphoserine (By similarity).
 MOD_RES 2751 2751 Phosphothreonine (By similarity).
 MOD_RES 2887 2887 Phosphoserine (By similarity).
 MOD_RES 2918 2918 Phosphoserine (By similarity).
 MOD_RES 3116 3116 Phosphoserine (By similarity).
 MOD_RES 3127 3127 Phosphoserine (By similarity).
 MOD_RES 3663 3663 Phosphoserine (By similarity).
 MOD_RES 3753 3753 Phosphoserine (By similarity).
 MOD_RES 3758 3758 Phosphoserine.
 MOD_RES 3761 3761 Phosphoserine (By similarity).
 MOD_RES 3810 3810 Phosphoserine.
 MOD_RES 3818 3818 Phosphoserine (By similarity).
 MOD_RES 3922 3922 Phosphoserine (By similarity).
 MOD_RES 3927 3927 Phosphothreonine (By similarity).
 MOD_RES 3930 3930 Phosphothreonine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Differentiation; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Ligase; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4377 AA 
Protein Sequence
MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE LYHWVDLLDR 60
FDGILADAGQ TVENMSWMLV CDRPEKEQLK MLLLAVLNFT ALLIEYSFSR HLYSSIEHLT 120
TLLASSDMQV VLAVLNLLYV FSKRSNYITR LGSDKRTPLL TRLQHLAESW GGKENGFGLA 180
ECCRDLQMLK YPPSATTLHF EFYADPGAEV KIEKRTTSNT LHYIHIEQLD KISESPSEIM 240
ESLTKMYSIP KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI 300
LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT GTASYHGFLP 360
VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG EALVSCGMME ALLKVIKFLG 420
DEQDQITFVT RAVRVVDLIT NLDMAAFQSH SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ 480
RPSTTQEGEE METDMDGVQC IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL 540
KHIISNAEYY GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV 600
LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD PLGDTASNLG 660
SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ KPSIQKADGT ATAPPPRSNH 720
AAEEASSEDE EEEEVQAMQS FNSAQQNETE PNQQVVGTEE RIPIPLMDYI LNVMKFVESI 780
LSNNTTDDHC QEFVNQKGLL PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV 840
LQEGLLQLDL ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH 900
AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV LLSLCTPNSL 960
PSGCEFGQAD MQKLVPKDEK AGTTQGGKRS DGEQDGTAGS MDASAQGLLE GIELDGDTLA 1020
PMETDEPSSS DSKGKSKITP AMAARIKQIK PLLSASSRLG RALAELFGLL VKLCVGSPVR 1080
QRRSHHAAST TTAPTPAARS TASALTKLLT KGLSWQPPPY TPTPRFRLTF FICSVGFTSP 1140
MLFDERKYPY HLMLQKFLCS GGHNALFETF NWALSMGGKV PVSEGLEHSD LPDGTGEFLD 1200
AWLMLVEKMV NPTTVLESPH SLPAKLPGGV QSFPQFSALR FLVVTQKAAF TCIKNLWNRK 1260
PLKVYGGRMA ESMLAILCHI LRGEPVIRER LSKEKEGSRG EEEAGQEEGG SRREPQVNQQ 1320
QLQQLMDMGF TREHAMEALL NTSTMEQATE YLLTHPPPII GGVVRDLSMS EEDQMMRAIA 1380
MSLGQDIPMD QRAESPEEVA CRKEEEERKA REKQEEEEAK CLEKFQDADP LEQDELHTFT 1440
DTMLPGCFHL LDELPDTVYR VCDLIMTAIK RNGADYRDMI LKQVVNQVWE AADVLIKAAL 1500
PLTTSDTKTV SEWISQMATL PQASNLATRI LLLTLLFEEL KLPCAWVVES SGILNVLIKL 1560
LEVVQPCLQA AKEQKEVQTP KWITPVLLLI DFYEKTAISS KRRAQMTKYL QSNSNNWRWF 1620
DDRSGRWCSY SASNNSTIDS AWKSGETSVR FTAGRRRYTV QFTTMVQVNE ETGNRRPVML 1680
TLLRVPRLSK NSKSSNGQEL EKTLEESKET DIKHKENKGN DIPLALESTN TEKEASLDET 1740
KIGEILIQGL TEDMVTVLIR ACVSMLGVPV DPDTLHATLR LCLRLTRDHK YAMMFAELKS 1800
TRMILNLTQS SGFNGFTPLV TLLLRHIIED PCTLRHTMEK VVRSAATSGA GSTTSGVVSG 1860
SLGSREINYI LRVLGPAACR NPDIFTEVAN CCIRIALPAP RGSGTASDDE FENLRIKGPN 1920
AVQLVKTTPL KPSSLPVIPD TIKEVIYDML NALAAYHAPE EADKSDPKPG GTTQEVGQLL 1980
QDMGDDVYQQ YRSLTRQSSD FDTQSGFSLN SQVFAADGAP AETSTTGTSQ GEASTPEETR 2040
EGKKDKEGDR TSEEGKQKSK GSKPLMPTST ILRLLAELVR SYVGIATLIA NYSYTVGQSE 2100
LIKEDCSVLA FVLDHLLPHT QNAEDKDTPA LARLFLASLA AAGSGTDAQV ALVNEVKAAL 2160
GRALAMAEST EKHARLQAVM CIISTIMESC PSTSSFYSSA TAKTQHNGMN NIIRLFLKKG 2220
LVNDLARVPH SLDLSSPNMA NTVNAALKPL ETLSRIVNQP SSLFGSKSAS SKNKSEQDAQ 2280
GASQDSSSHQ QDPGEPGEAE VQEEDHDVTQ TEVADGDIMD GEAETDSVVI AGQPEVLSSQ 2340
EMQVENELED LIDELLERDG GSGNSTIIVS RSGEDESQED VLMDEAPSNL SQASTLQANR 2400
EDSMNILDPE DEEEHTQEED SSGSNEDEDD SQDEEEEEEE DEEDDQEDDE GEEGDEDDDD 2460
DGSEMELDED YPDMNASPLV RFERFDREDD LIIEFDNMFS SATDIPPSPG NIPTTHPLMV 2520
RHADHSSLTL GSGSSTTRLT QGIGRSQRTL RQLTANTGHT IHVHYPGNRQ PNPPLILQRL 2580
LGPSAAADIL QLSSSLPLQS RGRARLLVGN DDVHIIARSD DELLDDFFHD QSTATSQAGT 2640
LSSIPTALTR WTEECKVLDA ESMHDCVSVV KVPIVNHLEF LRDEELEERR EKRRKQLAEE 2700
ETKIIDKGKE DKENRDQSAQ CTVTKTNDST EQNVSDGTPM PDSYPTTPSS TDAPTSESKE 2760
TLGTLQPSQQ QPALPPPPSL GEIPQELQSP AEEVANSTQL LMPIELEELG PTRPSGEAET 2820
TQMELSPAPT ITSLSPERAE DSDALTAVSS QLEGSPMDTS SLASCTLEEA VGDTPAAGSS 2880
EQPTAGSSTP GDAPSVVAEV QGRPDVSRES NQPPEDSSPP ASSESSSTRD SAVAISGADS 2940
RGILEEPLPS TSSEEEDPLA GISLPEGVDP SFLAALPDDI RREVLQNQLG IRPPTRSAPS 3000
SNSSAPAVVG NPGVTEVSPE FLAALPPAIQ EEVLAQQRAE QQRRELAQNA SSDTPMDPVT 3060
FIQTLPSDLR RSVLEDMEDS VLAVMPPDIA AEAQALRREQ EARQRQLMHE RLFGHSSTSA 3120
LSAILRSPAF TSRLSGNRGV QYTRLAVQRG GTFQMGGSSS HNRPSGSNVD TLLRLRGRLL 3180
LDHEALSCLL VLLFVDEPKL NTSRLHRVLR NLCYHAQTRH WVIRSLLSIL QRSSESELCI 3240
ETPKLSTSEE RGKKSSKSCA SSSHENRPLD LLHKMESKSS NQLSWLSVSM DAALGCRTNI 3300
FQIQRSGGRK HTEKHASSGS TVHIHPQAAP VVCRHVLDTL IQLAKVFPSH FTQQRTKETN 3360
CESDRERGSK QACSPCSSQS SSSGICTDFW DLLVKLDNMN VSRKGKNSVK SVPVSSGGEG 3420
ETSPHSLEAS PLGQLMNMLS HPVIRRSSLL TEKLLRLLSL ISIALPENKV SEVQTNSSNS 3480
GSSTAATSNT STTTTTTTTA TAPTPTPPAA TTPVTSAPAL VAATAISTIT VAASTTVTTP 3540
TTATTTVSTS TTKGSKSPAK VGEGGSGIDF KMVSSGLTEN QLQLSVEVLT SHSCSEEGLE 3600
DAANVLLQLS RGDSGTRDTV LKLLLNGARH LGYTLCKQIG TLLAELREYN LEQQRRAQCE 3660
TLSPDGLPEE QPQTTKLKGK MQSRFDMAEN VVIVASQKRP LGGRELQLPS MSMLTSKTST 3720
QKFFLRVLQV IIQLRDDTRR ANKKAKQTGR LGSSGLGSAS SIQAAVRQLE AEADAIIQMV 3780
REGQRARRQQ QAATSESSNQ SETSVRREES PMDVDQPSPS AQDTQSIVIS DGTPQGEKEK 3840
EEKPPELPLL SEQLSLDELW DMLGECLKEL EESHDQHAVL VLQPAVEAFF LVHATERESK 3900
PPVRDTRESQ LAHIKDEPPP LSPAPLTPAT PSSLDPFFSR EPSSMHISSS LPPDTQKFLR 3960
FAETHRTVLN QILRQSTTHL ADGPFAVLVD YIRVLDFDVK RKYFRQELER LDEGLRKEDM 4020
AVHVRRDHVF EDSYRELHRK SPEEMKNRLY IVFEGEEGQD AGGLLREWYM IISREMFNPM 4080
YALFRTSPGD RVTYTINPSS HCNPNHLSYF KFVGRIVAKA VYDNRLLECY FTRSFYKHIL 4140
GKSVRYTDME SEDYHFYQGL VYLLENDVST LGYDLTFSTE VQEFGVCEVR DLKPNGANIL 4200
VTEENKKEYV HLVCQMRMTG AIRKQLAAFL EGFYEIIPKR LISIFTEQEL ELLISGLPTI 4260
DIDDLKSNTE YHKYQSNSIQ IQWFWRALRS FDQADRAKFL QFVTGTSKVP LQGFAALEGM 4320
NGIQKFQIHR DDRSTDRLPS AHTCFNQLDL PAYESFEKLR HMLLLAIQEC SEGFGLA 4377 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0003677; F:DNA binding; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0006284; P:base-excision repair; ISS:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016574; P:histone ubiquitination; ISS:UniProtKB.
 GO:0006513; P:protein monoubiquitination; ISS:UniProtKB.
 GO:0000209; P:protein polyubiquitination; ISS:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. 
Interpro
 IPR016024; ARM-type_fold.
 IPR025527; DUF4414.
 IPR010309; E3_Ub_ligase_DUF908.
 IPR010314; E3_Ub_ligase_DUF913.
 IPR000569; HECT.
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR004170; WWE-dom. 
Pfam
 PF14377; DUF4414
 PF06012; DUF908
 PF06025; DUF913
 PF00632; HECT
 PF00627; UBA
 PF02825; WWE 
SMART
 SM00119; HECTc
 SM00165; UBA 
PROSITE
 PS50237; HECT
 PS50030; UBA
 PS50330; UIM
 PS50918; WWE 
PRINTS