UniProt Accession

 FSAA_ECOLI; P78055; P77855; Q9R3X3 

Genbank Protein ID

 D88188; U00096; AP009048 

Genbank Nucleotide ID

 BAA13552.1; AAC73912.2; BAA35513.2 

Protein Name

 Fructose-6-phosphate aldolase 1 

Protein Synonyms/Alias

 Fructose-6-phosphate aldolase A; FSAA 

Gene Name

 fsaA 

Gene Synonyms/Alias

 fsa; mipB; ybiZ; b0825; JW5109 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
37	SIIAAGKKPLDVVLP	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the reversible formation of fructose 6- phosphate from dihydroxyacetone (DHA) and D-glyceraldehyde 3- phosphate via an aldolization reaction. Can utilize several aldehydes as acceptor compounds in vitro, and hydroxyacetone (HA) or 1-hydroxy-butan-2-one as alternative donor substrate. Is also able to catalyze the direct stereoselective self-aldol addition of glycolaldehyde to furnish D-(-)-threose, and cross-aldol reactions of glycolaldehyde to other aldehyde acceptors. Is not able to cleave fructose, fructose 1-phosphate, glucose 6-phosphate, sedoheptulose 1,7-bisphosphate, xylulose 5-phosphate, ribulose 5- phosphate, and fructose 1,6-bisphosphate; cannot use dihydroxyacetone phosphate as donor compound nor D-glyceraldehyde as acceptor. Does not display transaldolase activity. 

Sequence Annotation

 ACT_SITE 85 85 Schiff-base intermediate with substrate.  

Keyword

 3D-structure; Carbohydrate metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Reference proteome; Schiff base. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 220 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0097023; F:fructose 6-phosphate aldolase activity; IDA:EcoCyc.
 GO:0006000; P:fructose metabolic process; IEA:HAMAP. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR023001; F6P_aldolase.
 IPR001585; Transaldolase.
 IPR004731; Transaldolase_3A/3B.
 IPR018225; Transaldolase_AS. 

Pfam

 PF00923; Transaldolase 

SMART

  

PROSITE

 PS01054; TRANSALDOLASE_1
 PS00958; TRANSALDOLASE_2 

PRINTS