| Tag | Content |
|---|
| CPLM ID | CPLM-003416 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ribosomal RNA large subunit methyltransferase M |
Protein Synonyms/Alias | 23S rRNA (cytidine2498-2'-O)-methyltransferase; 23S rRNA 2'-O-ribose methyltransferase RlmM |
Gene Name | rlmM |
Gene Synonyms/Alias | ygdE; b2806; JW2777; b1976 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 14 | LCRPGFEKECAAEIT | acetylation | [1] | | 99 | MLQGVVEKGGELRVE | acetylation | [1] | | 114 | VADTNESKELLKFCR | acetylation | [1] | | 118 | NESKELLKFCRKFTV | acetylation | [1] | | 122 | ELLKFCRKFTVPLRA | acetylation | [1] | | 179 | YMGIPRLKFPADAPS | acetylation | [1] | | 263 | WLREDGFKFRPTRSN | acetylation | [1] | | 281 | MVCDMVEKPAKVAAL | acetylation | [1] | | 306 | RETIFNLKLPMKKRY | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA. Modifies C2498 in naked 23S rRNA, but not in assembled 50S subunits or ribosomes. |
Sequence Annotation | REGION 221 224 S-adenosyl-L-methionine binding.
ACT_SITE 306 306 Proton acceptor (Probable).
BINDING 188 188 S-adenosyl-L-methionine.
BINDING 240 240 S-adenosyl-L-methionine.
BINDING 260 260 S-adenosyl-L-methionine.
BINDING 277 277 S-adenosyl-L-methionine. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Methyltransferase; Reference proteome; rRNA processing; S-adenosyl-L-methionine; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 366 AA |
Protein Sequence | MNKVVLLCRP GFEKECAAEI TDKAGQREIF GFARVKENAG YVIYECYQPD DGDKLIRELP 60
FSSLIFARQW FVVGELLQHL PPEDRITPIV GMLQGVVEKG GELRVEVADT NESKELLKFC 120
RKFTVPLRAA LRDAGVLANY ETPKRPVVHV FFIAPGCCYT GYSYSNNNSP FYMGIPRLKF 180
PADAPSRSTL KLEEAFHVFI PADEWDERLA NGMWAVDLGA CPGGWTYQLV KRNMWVYSVD 240
NGPMAQSLMD TGQVTWLRED GFKFRPTRSN ISWMVCDMVE KPAKVAALMA QWLVNGWCRE 300
TIFNLKLPMK KRYEEVSHNL AYIQAQLDEH GINAQIQARQ LYHDREEVTV HVRRIWAAVG 360
GRRDER 366 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0070677; F:rRNA (cytosine-2'-O-)-methyltransferase activity; IDA:EcoCyc. GO:0000453; P:enzyme-directed rRNA 2'-O-methylation; IMP:EcoCyc. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |