CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025575
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 KLHL8 protein 
Protein Synonyms/Alias
 Kelch-like protein 8 
Gene Name
 KLHL8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
41MFDPLTNKWMMKASMubiquitination[1]
135RYDPHLDKWIEVKEMubiquitination[1, 2, 3]
140LDKWIEVKEMGQRRAubiquitination[1, 3, 4]
182RYDPRSNKWDYVAALubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Kelch repeat; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 269 AA 
Protein Sequence
MNSRRRHVGV ISVEGKVYAV GGHDGNEHLG SMEMFDPLTN KWMMKASMNT KRRGIALASL 60
GGPIYAIGGL DDNTCFNDVE RYDIESDQWS TVAPMNTPRG GVGSVALVNH VYAVGGNDGM 120
ASLSSVERYD PHLDKWIEVK EMGQRRAGNG VSKLHGCLYV VGGFDDNSPL SSVERYDPRS 180
NKWDYVAALT TPRGGVGIAT VMGKIFAVGG HNGNAYLNTV EAFDPVLNRW ELVGSVSHCR 240
AGAGVAVCSC LTSQIRDVGH GSNNVVDCM 269 
Gene Ontology
  
Interpro
 IPR015916; Gal_Oxidase_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF01344; Kelch_1 
SMART
 SM00612; Kelch 
PROSITE
  
PRINTS