CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007915
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GTPase NPA3 
Protein Synonyms/Alias
 Essential PCL1-interacting ATPase 1 
Gene Name
 NPA3 
Gene Synonyms/Alias
 EPA1; YJR072C; J1821 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
31NSHLRAEKTPPYVINubiquitination[1]
179FNKTDVCKADFAKEWubiquitination[1]
264DEYDQYYKQEREKALubiquitination[1]
269YYKQEREKALNLKKKubiquitination[1]
345FPGEERTKGEVNENSubiquitination[1]
369EAMQQVGKTASSETAubiquitination[1, 2]
381ETAENIAKYIRN***ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 GTPase essential for viability. May have a role in mitotic exit. 
Sequence Annotation
 NP_BIND 10 17 GTP (Pootential).
 MOD_RES 304 304 Phosphoserine.
 MOD_RES 308 308 Phosphoserine.
 MOD_RES 313 313 Phosphoserine.
 MOD_RES 352 352 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; GTP-binding; Hydrolase; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 385 AA 
Protein Sequence
MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA NIDIRDSIKY 60
KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF QNCIIDTPGQ IECFVWSASG 120
AIITESFASS FPTVIAYIVD TPRNSSPTTF MSNMLYACSI LYKTKLPMIV VFNKTDVCKA 180
DFAKEWMTDF ESFQAAIKED QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF 240
TGDGFDEFMQ CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS 300
AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE NSAPDLQRRY 360
QEAMQQVGKT ASSETAENIA KYIRN 385 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IMP:SGD.
 GO:0007064; P:mitotic sister chromatid cohesion; IMP:SGD.
 GO:0044376; P:RNA polymerase II complex localization to nucleus; IMP:SGD. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR004130; Uncharacterised_ATP-bd. 
Pfam
 PF03029; ATP_bind_1 
SMART
  
PROSITE
  
PRINTS