CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019571
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PHD finger protein 12 
Protein Synonyms/Alias
 PHD factor 1; Pf1 
Gene Name
 PHF12 
Gene Synonyms/Alias
 KIAA1523 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
751MLDEKLIKFLALQRIubiquitination[1, 2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA. 
Sequence Annotation
 DOMAIN 815 869 FHA.
 ZN_FING 56 105 PHD-type 1.
 ZN_FING 271 321 PHD-type 2; atypical.
 REGION 102 273 Interaction with SIN3A.
 REGION 105 128 PBR.
 REGION 300 704 Interaction with SIN3A.
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 586 586 Phosphothreonine.
 MOD_RES 671 671 Phosphothreonine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1004 AA 
Protein Sequence
MWEKMETKTI VYDLDTSGGL MEQIQALLAP PKTDEAEKRS RKPEKEPRRS GRATNHDSCD 60
SCKEGGDLLC CDHCPAAFHL QCCNPPLSEE MLPPGEWMCH RCTVRRKKRE QKKELGHVNG 120
LVDKSGKRTT SPSSDTDLLD RSASKTELKA IAHARILERR ASRPGTPTSS ASTETPTSEQ 180
NDVDEDIIDV DEEPVAAEPD YVQPQLRRPF ELLIAAAMER NPTQFQLPNE LTCTTALPGS 240
SKRRRKEETT GKNVKKTQHE LDHNGLVPLP VKVCFTCNRS CRVAPLIQCD YCPLLFHMDC 300
LEPPLTAMPL GRWMCPNHIE HVVLNQKNMT LSNRCQVFDR FQDTVSQHVV KVDFLNRIHK 360
KHPPNRRVLQ SVKRRSLKVP DAIKSQYQFP PPLIAPAAIR DGELICNGIP EESQMHLLNS 420
EHLATQAEQQ EWLCSVVALQ CSILKHLSAK QMPSHWDSEQ TEKADIKPVI VTDSSVTTSL 480
QTADKTPTPS HYPLSCPSGI STQNSLSCSP PHQSPALEDI GCSSCAEKSK KTPCGTANGP 540
VNTEVKANGP HLYSSPTDST DPRRLPGANT PLPGLSHRQG WPRPLTPPAA GGLQNHTVGI 600
IVKTENATGP SSCPQRSLVP VPSLPPSIPS SCASIENTST LQRKTVQSQI GPPLTDSRPL 660
GSPPNATRVL TPPQAAGDGI LATTANQRFS SPAPSSDGKV SPGTLSIGSA LTVPSFPANS 720
TAMVDLTNSL RAFMDVNGEI EINMLDEKLI KFLALQRIHQ LFPSRVQPSP GSVGTHQLAS 780
GGHHIEVQRK EVQARAVFYP LLGLGGAVNM CYRTLYIGTG ADMDVCLTNY GHCNYVSGKH 840
ACIFYDENTK HYELLNYSEH GTTVDNVLYS CDFSEKTPPT PPSSIVAKVQ SVIRRRRHQK 900
QDEEPSEEAA MMSSQAQGPQ RRPCNCKASS SSLIGGSGAG WEGTALLHHG SYIKLGCLQF 960
VFSITEFATK QPKGDASLLQ DGVLAEKLSL KPHQGPVLRS NSVP 1004 
Gene Ontology
 GO:0016580; C:Sin3 complex; IDA:MGI.
 GO:0017053; C:transcriptional repressor complex; IDA:UniProtKB.
 GO:0035091; F:phosphatidylinositol binding; IDA:UniProtKB.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IDA:MGI.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR000253; FHA_dom.
 IPR008984; SMAD_FHA_domain.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR019787; Znf_PHD-finger.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00628; PHD 
SMART
 SM00249; PHD 
PROSITE
 PS50006; FHA_DOMAIN
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2 
PRINTS