CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007542
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Septin-2 
Protein Synonyms/Alias
 Neural precursor cell expressed developmentally down-regulated protein 5; NEDD-5 
Gene Name
 Sept2 
Gene Synonyms/Alias
 Nedd-5; Nedd5 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
3*****MSKQQPTQFIubiquitination[1]
74IIPGAAEKIERTVQIubiquitination[1]
183NIVPVIAKADTLTLKubiquitination[1]
225SDEDEDFKEQTRLLKubiquitination[1]
232KEQTRLLKASIPFSVubiquitination[1]
249SNQLIEAKGKKVRGRubiquitination[1]
318VENEDMNKDQILLEKubiquitination[1]
325KDQILLEKEAELRRMubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements (By similarity). In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. 
Sequence Annotation
 NP_BIND 44 52 GTP.
 NP_BIND 183 186 GTP.
 REGION 260 270 Important for dimerization (By
 BINDING 78 78 GTP.
 BINDING 104 104 GTP; via amide nitrogen.
 BINDING 241 241 GTP; via amide nitrogen and carbonyl
 BINDING 256 256 GTP.
 BINDING 258 258 GTP (By similarity).
 MOD_RES 190 190 N6-acetyllysine (By similarity).
 MOD_RES 218 218 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Cell cycle; Cell division; Cell membrane; Cell projection; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Kinetochore; Membrane; Mitosis; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 361 AA 
Protein Sequence
MSKQQPTQFI NPETPGYVGF ANLPNQVHRK SVKKGFEFTL MVVGESGLGK STLINSLFLT 60
DLYPERIIPG AAEKIERTVQ IEASTVEIEE RGVKLRLTVV DTPGYGDAIN CRDCFKTIIS 120
YIDEQFERYL HDESGLNRRH IIDNRVHCCF YFISPFGHGL KPLDVAFMKA IHNKVNIVPV 180
IAKADTLTLK ERERLKKRIL DEIEEHSIKI YHLPDAESDE DEDFKEQTRL LKASIPFSVV 240
GSNQLIEAKG KKVRGRLYPW GVVEVENPEH NDFLKLRTML ITHMQDLQEV TQDLHYENFR 300
SERLKRGGRK VENEDMNKDQ ILLEKEAELR RMQEMIARMQ AQMQMQMQGG DSDSGALGQH 360
V 361 
Gene Ontology
 GO:0005826; C:actomyosin contractile ring; NAS:UniProtKB.
 GO:0009986; C:cell surface; IDA:MGI.
 GO:0060170; C:cilium membrane; IDA:UniProtKB.
 GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0000145; C:exocyst; IEA:Compara.
 GO:0030496; C:midbody; TAS:MGI.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0031105; C:septin complex; IEA:InterPro.
 GO:0005876; C:spindle microtubule; TAS:MGI.
 GO:0045202; C:synapse; IDA:MGI.
 GO:0030234; F:enzyme regulator activity; IDA:MGI.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; NAS:UniProtKB.
 GO:0042802; F:identical protein binding; TAS:MGI.
 GO:0032947; F:protein complex scaffold; IDA:MGI.
 GO:0042384; P:cilium assembly; IMP:UniProtKB.
 GO:0000910; P:cytokinesis; NAS:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0031175; P:neuron projection development; IEA:Compara.
 GO:0051258; P:protein polymerization; TAS:MGI.
 GO:0002036; P:regulation of L-glutamate transport; IDA:MGI.
 GO:0032880; P:regulation of protein localization; IDA:MGI.
 GO:0007224; P:smoothened signaling pathway; IMP:UniProtKB. 
Interpro
 IPR000038; Cell_div_GTP-bd.
 IPR027417; P-loop_NTPase.
 IPR016491; Septin.
 IPR008113; Septin2. 
Pfam
 PF00735; Septin 
SMART
  
PROSITE
  
PRINTS
 PR01740; SEPTIN2.