UniProt Accession

 VDAC1_MOUSE; Q60932; B1ASZ9; Q5SVC6 

Genbank Protein ID

 U30840; AK168672; AK169671; AK169354; AK169282; AK169160; AL645589; AL645589; BC092257 

Genbank Nucleotide ID

 AAB47777.1; BAE40522.1; BAE41292.1; BAE41103.1; BAE41040.1; BAE40939.1; CAI24939.1; CAM19812.1; AAH92257.1 

Protein Name

 Voltage-dependent anion-selective channel protein 1 

Protein Synonyms/Alias

 VDAC-1; mVDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Voltage-dependent anion-selective channel protein 5; VDAC-5; mVDAC5 

Gene Name

 Vdac1 

Gene Synonyms/Alias

 Vdac5 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
33	SARDVFTKGYGFGLI	acetylation	[1, 2, 3]
33	SARDVFTKGYGFGLI	ubiquitination	[4]
41	GYGFGLIKLDLKTKS	acetylation	[1, 2, 3]
41	GYGFGLIKLDLKTKS	ubiquitination	[4]
45	GLIKLDLKTKSENGL	acetylation	[3]
47	IKLDLKTKSENGLEF	acetylation	[3]
47	IKLDLKTKSENGLEF	ubiquitination	[4]
66	SANTETTKVNGSLET	ubiquitination	[4]
74	VNGSLETKYRWTEYG	acetylation	[1, 2, 3]
74	VNGSLETKYRWTEYG	ubiquitination	[4]
109	DQLARGLKLTFDSSF	acetylation	[3]
122	SFSPNTGKKNAKIKT	ubiquitination	[4]
210	FGGSIYQKVNKKLET	acetylation	[3]
210	FGGSIYQKVNKKLET	ubiquitination	[4]
237	TRFGIAAKYQVDPDA	acetylation	[1, 2, 3, 5, 6, 7, 8, 9, 10]
249	PDACFSAKVNNSSLI	acetylation	[7, 8, 9, 10]
249	PDACFSAKVNNSSLI	succinylation	[9]
265	LGYTQTLKPGIKLTL	acetylation	[3, 6, 8, 10, 11]
265	LGYTQTLKPGIKLTL	ubiquitination	[4]
279	LSALLDGKNVNAGGH	acetylation	[3, 5, 8, 10, 11]
279	LSALLDGKNVNAGGH	ubiquitination	[4]
287	NVNAGGHKLGLGLEF	acetylation	[11]
287	NVNAGGHKLGLGLEF	ubiquitination	[4]

Reference

 [1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [2] Proteomics of mitochondrial inner and outer membranes.
 Distler AM, Kerner J, Hoppel CL.
 Proteomics. 2008 Oct;8(19):4066-82. [PMID: 18763707]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [8] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [9] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [10] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [11] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 

Functional Description

 Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. 

Sequence Annotation

 NP_BIND 255 257 NAD (By similarity).
 NP_BIND 273 277 NAD (By similarity).
 MOD_RES 14 14 N-acetylmethionine; in isoform Mt-VDAC1
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 33 33 N6-acetyllysine.
 MOD_RES 41 41 N6-acetyllysine.
 MOD_RES 74 74 N6-acetyllysine.
 MOD_RES 80 80 Phosphotyrosine.
 MOD_RES 117 117 Phosphoserine.
 MOD_RES 208 208 Phosphotyrosine.
 MOD_RES 237 237 N6-acetyllysine.
 MOD_RES 279 279 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Alternative splicing; Apoptosis; Cell membrane; Complete proteome; Direct protein sequencing; Ion transport; Membrane; Mitochondrion; Mitochondrion outer membrane; NAD; Nucleotide-binding; Phosphoprotein; Porin; Reference proteome; Transmembrane; Transmembrane beta strand; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 296 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0042645; C:mitochondrial nucleoid; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; ISO:MGI.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0046930; C:pore complex; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0015288; F:porin activity; IEA:UniProtKB-KW.
 GO:0008308; F:voltage-gated anion channel activity; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0001662; P:behavioral fear response; IMP:MGI.
 GO:0007612; P:learning; IMP:MGI.
 GO:0007270; P:neuron-neuron synaptic transmission; IMP:MGI. 

Interpro

 IPR023614; Porin_dom.
 IPR001925; Porin_Euk.
 IPR027246; Porin_Euk/Tom40. 

Pfam

 PF01459; Porin_3 

SMART

  

PROSITE

 PS00558; EUKARYOTIC_PORIN 

PRINTS

 PR00185; EUKARYTPORIN.