CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012637
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intersectin-1 
Protein Synonyms/Alias
 SH3 domain-containing protein 1A; SH3P17 
Gene Name
 ITSN1 
Gene Synonyms/Alias
 ITSN; SH3D1A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
472QEDIVVLKAKKKTLEacetylation[1]
495KKHQLEGKLQDIRCRacetylation[2]
608DIFNNQLKELREIHNubiquitination[3]
658RRAQERDKQWLEHVQubiquitination[4, 5]
826STSAPAPKLALRETPubiquitination[3]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1. 
Sequence Annotation
 DOMAIN 21 109 EH 1.
 DOMAIN 53 88 EF-hand 1.
 DOMAIN 221 310 EH 2.
 DOMAIN 254 289 EF-hand 2.
 DOMAIN 740 806 SH3 1.
 DOMAIN 913 971 SH3 2.
 DOMAIN 1002 1060 SH3 3.
 DOMAIN 1074 1138 SH3 4.
 DOMAIN 1155 1214 SH3 5.
 DOMAIN 1237 1423 DH.
 DOMAIN 1462 1571 PH.
 DOMAIN 1583 1679 C2.
 REGION 326 702 KLERQ.
 MOD_RES 313 313 Phosphoserine (By similarity).
 MOD_RES 315 315 Phosphoserine (By similarity).
 MOD_RES 318 318 Phosphoserine (By similarity).
 MOD_RES 902 902 Phosphoserine.
 MOD_RES 904 904 Phosphoserine.
 MOD_RES 977 977 Phosphothreonine (By similarity).
 MOD_RES 1137 1137 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Calcium; Cell junction; Cell projection; Coated pit; Coiled coil; Complete proteome; Endocytosis; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; SH3 domain; Synapse; Synaptosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1721 AA 
Protein Sequence
MAQFPTPFGG SLDIWAITVE ERAKHDQQFH SLKPISGFIT GDQARNFFFQ SGLPQPVLAQ 60
IWALADMNND GRMDQVEFSI AMKLIKLKLQ GYQLPSALPP VMKQQPVAIS SAPAFGMGGI 120
ASMPPLTAVA PVPMGSIPVV GMSPTLVSSV PTAAVPPLAN GAPPVIQPLP AFAHPAATLP 180
KSSSFSRSGP GSQLNTKLQK AQSFDVASVP PVAEWAVPQS SRLKYRQLFN SHDKTMSGHL 240
TGPQARTILM QSSLPQAQLA SIWNLSDIDQ DGKLTAEEFI LAMHLIDVAM SGQPLPPVLP 300
PEYIPPSFRR VRSGSGISVI SSTSVDQRLP EEPVLEDEQQ QLEKKLPVTF EDKKRENFER 360
GNLELEKRRQ ALLEQQRKEQ ERLAQLERAE QERKERERQE QERKRQLELE KQLEKQRELE 420
RQREEERRKE IERREAAKRE LERQRQLEWE RNRRQELLNQ RNKEQEDIVV LKAKKKTLEF 480
ELEALNDKKH QLEGKLQDIR CRLTTQRQEI ESTNKSRELR IAEITHLQQQ LQESQQMLGR 540
LIPEKQILND QLKQVQQNSL HRDSLVTLKR ALEAKELARQ HLRDQLDEVE KETRSKLQEI 600
DIFNNQLKEL REIHNKQQLQ KQKSMEAERL KQKEQERKII ELEKQKEEAQ RRAQERDKQW 660
LEHVQQEDEH QRPRKLHEEE KLKREESVKK KDGEEKGKQE AQDKLGRLFH QHQEPAKPAV 720
QAPWSTAEKG PLTISAQENV KVVYYRALYP FESRSHDEIT IQPGDIVMVK GEWVDESQTG 780
EPGWLGGELK GKTGWFPANY AEKIPENEVP APVKPVTDST SAPAPKLALR ETPAPLAVTS 840
SEPSTTPNNW ADFSSTWPTS TNEKPETDNW DAWAAQPSLT VPSAGQLRQR SAFTPATATG 900
SSPSPVLGQG EKVEGLQAQA LYPWRAKKDN HLNFNKNDVI TVLEQQDMWW FGEVQGQKGW 960
FPKSYVKLIS GPIRKSTSMD SGSSESPASL KRVASPAAKP VVSGEEFIAM YTYESSEQGD 1020
LTFQQGDVIL VTKKDGDWWT GTVGDKAGVF PSNYVRLKDS EGSGTAGKTG SLGKKPEIAQ 1080
VIASYTATGP EQLTLAPGQL ILIRKKNPGG WWEGELQARG KKRQIGWFPA NYVKLLSPGT 1140
SKITPTEPPK STALAAVCQV IGMYDYTAQN DDELAFNKGQ IINVLNKEDP DWWKGEVNGQ 1200
VGLFPSNYVK LTTDMDPSQQ WCSDLHLLDM LTPTERKRQG YIHELIVTEE NYVNDLQLVT 1260
EIFQKPLMES ELLTEKEVAM IFVNWKELIM CNIKLLKALR VRKKMSGEKM PVKMIGDILS 1320
AQLPHMQPYI RFCSRQLNGA ALIQQKTDEA PDFKEFVKRL AMDPRCKGMP LSSFILKPMQ 1380
RVTRYPLIIK NILENTPENH PDHSHLKHAL EKAEELCSQV NEGVREKENS DRLEWIQAHV 1440
QCEGLSEQLV FNSVTNCLGP RKFLHSGKLY KAKSNKELYG FLFNDFLLLT QITKPLGSSG 1500
TDKVFSPKSN LQYKMYKTPI FLNEVLVKLP TDPSGDEPIF HISHIDRVYT LRAESINERT 1560
AWVQKIKAAS ELYIETEKKK REKAYLVRSQ RATGIGRLMV NVVEGIELKP CRSHGKSNPY 1620
CEVTMGSQCH ITKTIQDTLN PKWNSNCQFF IRDLEQEVLC ITVFERDQFS PDDFLGRTEI 1680
RVADIKKDQG SKGPVTKCLL LHEVPTGEIV VRLDLQLFDE P 1721 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005905; C:coated pit; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030139; C:endocytic vesicle; IEA:Compara.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0043005; C:neuron projection; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0045202; C:synapse; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; NAS:UniProtKB.
 GO:0019209; F:kinase activator activity; IEA:Compara.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0032947; F:protein complex scaffold; IDA:UniProtKB.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0048488; P:synaptic vesicle endocytosis; TAS:ProtInc. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR000219; DH-domain.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR001331; GDS_CDC24_CS.
 IPR026813; ITSN1.
 IPR000108; p67phox.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00168; C2
 PF00169; PH
 PF00621; RhoGEF
 PF00018; SH3_1
 PF07653; SH3_2 
SMART
 SM00239; C2
 SM00054; EFh
 SM00027; EH
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS50004; C2
 PS00741; DH_1
 PS50010; DH_2
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.