CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000551
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit beta' 
Protein Synonyms/Alias
 Beta'-coat protein; Beta'-COP; p102 
Gene Name
 Copb2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
8MPLRLDIKRKLTAMSacetylation[1]
608RDFSMADKVLPTIPKubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). 
Sequence Annotation
 REPEAT 13 52 WD 1.
 REPEAT 55 94 WD 2.
 REPEAT 97 136 WD 3.
 REPEAT 140 180 WD 4.
 REPEAT 183 224 WD 5.
 REPEAT 227 266 WD 6.
 REPEAT 350 388 WD 7.
 REPEAT 390 425 WD 8.
 REPEAT 746 783 WD 9.
 MOD_RES 627 627 N6-acetyllysine (By similarity).
 MOD_RES 859 859 Phosphoserine (By similarity).  
Keyword
 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 905 AA 
Protein Sequence
MPLRLDIKRK LTAMSDRVKS VDLHPTEPWM LASLYNGSVC VWNHETQTLV KTFEVCDLPV 60
RAAKFVARKN WVVTGADDMQ IRVFNYNTLE RVHMFEAHSD YIRCIAVHPT QPFILTSSDD 120
MLIKLWDWDK KWSCSQVFEG HTHYVMQIVI NPKDNNQFAS ASLDRTIKVW QLGSSSPNFT 180
LEGHEKGVNC IDYYSGGDKP YLISGADDRL VKIWDYQNKT CVQTPEGHAQ NVSCATFHPE 240
LPIIITGSED GTVRIWHSST YRLESTLNYG MERVWCVASL RGSNNVALGY DEGSIIVKLG 300
REEPAMSMDA NGKIIWAKHS EVQQANLKAM GDTEIKDGER LPLAVKDMGS CEIYPQTIQH 360
NPNGRFVVVC GDGEYIIYTA MALRNKSFGS AQEFAWAHDS SEYAIRESNS VVKIFKNFKE 420
KKSFKPDFGA ESIYGGFLLG VRSVNGLAFY DWENTELIRR IEIQPKHIFW SDSGELVCIA 480
TEESFFILKY LSEKVLAAQE THEGVTEDGI EDAFEVLGEI QEIVKTGLWV GDCFIYTSSV 540
NRLNYYVGGE IVTIAHLDRT MYLLGYIPKD NRLYLGDKEL NIVSYSLLVS VLEYQTAVMR 600
RDFSMADKVL PTIPKEQRTR VAHFLEKQGF KQQALTVSTD PEHRFELALQ LGELKIAYQL 660
AVEAESEQKW KQLAELAISK CQFSLAQECL HHAQDYGGLL LLATASGNAS MVNKLAEGAE 720
RDGKNNVAFM SYFLQGKLDA CLELLIRTGR LPEAAFLART YLPSQVSRVV KLWRENLSKV 780
NQKAAESLAD PTEYENLFPG LKEAFVVEEW VKETHADLWP AKQYPLVTPN EERNVMEEAK 840
RFQPSRATAQ QEPDGKPASS PVIMASQTTH KEEKSFQELE DDLDTMELED IDTTDINLDE 900
DILDD 905 
Gene Ontology
 GO:0030663; C:COPI-coated vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; IDA:RGD.
 GO:0030117; C:membrane coat; IEA:InterPro.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 
Interpro
 IPR006692; Coatomer_WD-assoc_reg.
 IPR016453; COPB2.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04053; Coatomer_WDAD
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.