CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001079
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Estradiol 17-beta-dehydrogenase 12 
Protein Synonyms/Alias
 17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase; KAR; KIK-I 
Gene Name
 Hsd17b12 
Gene Synonyms/Alias
 Kik1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
72AYAEELAKRGMKIVLubiquitination[1]
86LISRSQDKLNQVSNNacetylation[2, 3]
95NQVSNNIKEKFNVETubiquitination[1]
97VSNNIKEKFNVETRTacetylation[2]
117SLDDIYDKIKTGLSGubiquitination[1]
221QCLHEEYKSKGIFVQubiquitination[1]
223LHEEYKSKGIFVQSVubiquitination[1]
238MPYLVATKLAKIQKPubiquitination[1]
244TKLAKIQKPTLDKPSacetylation[2, 3]
249IQKPTLDKPSAETFVacetylation[2, 3, 4]
261TFVKSAIKTVGLQTRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in females. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation (By similarity). 
Sequence Annotation
 NP_BIND 50 79 NADP (By similarity).
 MOTIF 308 312 Di-lysine motif (By similarity).
 ACT_SITE 202 202 Proton acceptor (By similarity).
 BINDING 189 189 Substrate (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Endoplasmic reticulum; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Reference proteome; Steroid biosynthesis; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 312 AA 
Protein Sequence
MECAPPAAGF LYWVGASTIA YLALRASYSL FRAFQVWCVG NEALVGPRLG EWAVVTGGTD 60
GIGKAYAEEL AKRGMKIVLI SRSQDKLNQV SNNIKEKFNV ETRTIAVDFS LDDIYDKIKT 120
GLSGLEIGVL VNNVGMSYEY PEYFLEIPDL DNTIKKLINI NVLSVCKVTR LVLPGMVERS 180
KGVILNISSA SGMLPVPLLT IYSATKAFVD FFSQCLHEEY KSKGIFVQSV MPYLVATKLA 240
KIQKPTLDKP SAETFVKSAI KTVGLQTRTT GYVIHSLMGS INSIMPRWMY FKIIMGFSKS 300
LRNRYLKKRK KN 312 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0031012; C:extracellular matrix; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005518; F:collagen binding; IDA:MGI.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:EC.
 GO:0001968; F:fibronectin binding; IDA:MGI.
 GO:0008201; F:heparin binding; IDA:MGI.
 GO:0006703; P:estrogen biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0030198; P:extracellular matrix organization; IDA:MGI.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0010811; P:positive regulation of cell-substrate adhesion; IDA:MGI. 
Interpro
 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 
Pfam
 PF00106; adh_short 
SMART
  
PROSITE
 PS00061; ADH_SHORT 
PRINTS
 PR00081; GDHRDH.
 PR00080; SDRFAMILY.