CPLM-022416

Genbank Nucleotide ID

Ubiquitin-associated protein 1

Protein Synonyms/Alias

UBAP-1; Nasopharyngeal carcinoma-associated gene 20 protein

Homo sapiens (Human)

Position	Peptide	Type	References
89	KIAEAEAKVNSKSGP	ubiquitination	[1, 2]
93	AEAKVNSKSGPEGDS	ubiquitination	[1]
101	SGPEGDSKMSFSKTH	ubiquitination	[2]
177	ELKTIDEKEELRNIL	ubiquitination	[1, 2, 3, 4]
251	EKMSLSSKVSLPPIP	ubiquitination	[2]
264	IPAVSNIKSLSFPKL	ubiquitination	[2, 4, 5, 6, 7]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Plays a role in the proteasomal degradation of ubiquitinated cell-surface proteins, such as EGFR and BST2.

DOMAIN 17 63 UMA.
DOMAIN 389 430 UBA 1.
DOMAIN 451 498 UBA 2.

3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Endosome; Polymorphism; Protein transport; Reference proteome; Repeat; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:UniProtKB.
GO:0000813; C:ESCRT I complex; IDA:UniProtKB.
GO:0043130; F:ubiquitin binding; IDA:UniProtKB.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:UniProtKB.

IPR009060; UBA-like.
IPR015940; UBA/transl_elong_EF1B_N_euk.
IPR023340; UMA.

PS50030; UBA
PS51497; UMA