CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031393
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar GTP-binding protein 1 
Protein Synonyms/Alias
 cDNA FLJ56266, highly similar to Nucleolar GTP-binding protein 1 
Gene Name
 GTPBP4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
26GRMCTVIKRQKQSLEubiquitination[1]
29CTVIKRQKQSLEYLEubiquitination[1, 2]
65CGYPNVGKSSFINKVubiquitination[1, 3, 4, 5, 6]
71GKSSFINKVTRADVDubiquitination[1, 6]
87QPYAFTTKSLFVGHMubiquitination[1, 2]
97FVGHMDYKYLRWQVVubiquitination[1]
166IRPLFINKPLIVVANubiquitination[1]
255IPTRRDDKERPPFIPubiquitination[1]
333IDPAIMKKLEELEKEubiquitination[1, 6]
378IREKKKLKILESKEKubiquitination[1]
406VQRTVLEKEMRSLGVacetylation[7]
406VQRTVLEKEMRSLGVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; GTP-binding; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 518 AA 
Protein Sequence
MKYGDSLYRC KQLKRAALGR MCTVIKRQKQ SLEYLEQVRQ HLSRLPTIDP NTRTLLLCGY 60
PNVGKSSFIN KVTRADVDVQ PYAFTTKSLF VGHMDYKYLR WQVVDTPGIL DHPLEDRNTI 120
EMQAITALAH LRAAVLYVMD LSEQCGHGLR EQLELFQNIR PLFINKPLIV VANKCDVKRI 180
AELSEDDQKI FTDLQSEGFP VIETSTLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV 240
LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERDLELEMG DDYILDLQKY 300
WDLMNLSEKH DKIPEIWEGH NIADYIDPAI MKKLEELEKE EELRTAAGEY DSVSESEDEE 360
MLEIRQLAKQ IREKKKLKIL ESKEKNTQGP RMPRTAKKVQ RTVLEKEMRS LGVDMDDKDD 420
AHYAVQARRS RSITRKRKRE DSAPPSSVAR SGSCSRTPRD VSGLRDVKMV KKAKTMMKNA 480
QKKMNRLGKK GEADRHVFDM KPKHLLSGKR KAGKKDRR 518 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW. 
Interpro
 IPR006073; GTP_binding_domain.
 IPR010674; NOG1_Rossman_fold_dom.
 IPR012973; NOG_C.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom. 
Pfam
 PF06858; NOG1
 PF08155; NOGCT 
SMART
  
PROSITE
  
PRINTS
 PR00326; GTP1OBG.