CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018838
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histone acetyltransferase KAT2A 
Protein Synonyms/Alias
 General control of amino acid synthesis protein 5-like 2; Histone acetyltransferase GCN5; HsGCN5; Lysine acetyltransferase 2A; STAF97 
Gene Name
 KAT2A 
Gene Synonyms/Alias
 GCN5; GCN5L2; HGCN5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
289AEDVATYKVNYTRWLubiquitination[1, 2]
446NLTLEDAKRLRVMGDubiquitination[3, 4]
555HKTLALIKDGRVIGGubiquitination[3]
688RKQAQIRKVYPGLSCubiquitination[3]
776IRFPIDLKTMTERLRubiquitination[4]
791SRYYVTRKLFVADLQubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. 
Sequence Annotation
 DOMAIN 503 656 N-acetyltransferase.
 DOMAIN 745 815 Bromo.  
Keyword
 3D-structure; Alternative splicing; Bromodomain; Complete proteome; Host-virus interaction; Nucleus; Reference proteome; Transcription; Transcription regulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 837 AA 
Protein Sequence
MAEPSQAPTP APAAQPRPLQ SPAPAPTPTP APSPASAPIP TPTPAPAPAP AAAPAGSTGT 60
GGPGVGSGGA GSGGDPARPG LSQQQRASQR KAQVRGLPRA KKLEKLGVFS ACKANETCKC 120
NGWKNPKPPT APRMDLQQPA ANLSELCRSC EHPLADHVSH LENVSEDEIN RLLGMVVDVE 180
NLFMSVHKEE DTDTKQVYFY LFKLLRKCIL QMTRPVVEGS LGSPPFEKPN IEQGVLNFVQ 240
YKFSHLAPRE RQTMFELSKM FLLCLNYWKL ETPAQFRQRS QAEDVATYKV NYTRWLCYCH 300
VPQSCDSLPR YETTHVFGRS LLRSIFTVTR RQLLEKFRVE KDKLVPEKRT LILTHFPKFL 360
SMLEEEIYGA NSPIWESGFT MPPSEGTQLV PRPASVSAAV VPSTPIFSPS MGGGSNSSLS 420
LDSAGAEPMP GEKRTLPENL TLEDAKRLRV MGDIPMELVN EVMLTITDPA AMLGPETSLL 480
SANAARDETA RLEERRGIIE FHVIGNSLTP KANRRVLLWL VGLQNVFSHQ LPRMPKEYIA 540
RLVFDPKHKT LALIKDGRVI GGICFRMFPT QGFTEIVFCA VTSNEQVKGY GTHLMNHLKE 600
YHIKHNILYF LTYADEYAIG YFKKQGFSKD IKVPKSRYLG YIKDYEGATL MECELNPRIP 660
YTELSHIIKK QKEIIKKLIE RKQAQIRKVY PGLSCFKEGV RQIPVESVPG IRETGWKPLG 720
KEKGKELKDP DQLYTTLKNL LAQIKSHPSA WPFMEPVKKS EAPDYYEVIR FPIDLKTMTE 780
RLRSRYYVTR KLFVADLQRV IANCREYNPP DSEYCRCASA LEKFFYFKLK EGGLIDK 837 
Gene Ontology
 GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IDA:BHF-UCL.
 GO:0072686; C:mitotic spindle; IEA:Compara.
 GO:0030914; C:STAGA complex; IDA:UniProtKB.
 GO:0033276; C:transcription factor TFTC complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0010484; F:H3 histone acetyltransferase activity; IDA:BHF-UCL.
 GO:0043997; F:histone acetyltransferase activity (H4-K12 specific); IEA:Compara.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0006338; P:chromatin remodeling; TAS:ProtInc.
 GO:0016578; P:histone deubiquitination; IDA:UniProtKB.
 GO:0044154; P:histone H3-K14 acetylation; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0022037; P:metencephalon development; IEA:Compara.
 GO:0030901; P:midbrain development; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0001843; P:neural tube closure; IEA:Compara.
 GO:0042981; P:regulation of apoptotic process; IEA:Compara.
 GO:0031647; P:regulation of protein stability; IMP:MGI.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0001756; P:somitogenesis; IEA:Compara.
 GO:0021537; P:telencephalon development; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR000182; GNAT_dom.
 IPR016376; Hist_acetylase_PCAF.
 IPR009464; PCAF_N. 
Pfam
 PF00583; Acetyltransf_1
 PF00439; Bromodomain
 PF06466; PCAF_N 
SMART
 SM00297; BROMO 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51186; GNAT 
PRINTS
 PR00503; BROMODOMAIN.